携带SPK1基因的重组腺病毒对犬酒精性胃溃疡愈合的影响  

Effect of sphingosine kinase-1 gene recombinant adenovirus on healing of gastric ulcer induced by alcohol in experimental dogs

作　　者：郭强[1] 张凯[2] 孔庆龙[3] 唐平[4] 张修礼[4] 孙刚[4] 

机构地区：[1]解放军总医院医疗处,北京100853 [2]解放军总医院基础医学研究所生物化学研究室,北京100853 [3]解放军总医院肿瘤外科,北京100853 [4]解放军总医院消化内科,北京100853

出　　处：《军医进修学院学报》2011年第5期496-498,500,共4页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金基金项目(30901462)~~

摘　　要：目的探讨基因药物Ad-SPK1对酒精性胃溃疡的治疗作用。方法建立酒精诱导的实验犬胃溃疡模型,制备携带绿色荧光蛋白(GFP)基因的腺病毒作为对照,观察GFP在犬胃壁中的分布,分别于给药后7、14、21、28d电子胃镜下观察溃疡的愈合情况,取胃溃疡组织进行Western blot检测SPK1蛋白的表达和γ-P32掺入法检测SPK酶活性和免疫组化检测CD34表达和MVD计数。结果腺病毒能有效介导GFP和SPK1基因在胃溃疡黏膜层细胞表达。电子内镜下可见腺病毒介导的SPK1基因转移能促进胃溃疡愈合。微血管密度计数结果显示,Ad-SPK1组的微血管密度计数值明显高于对照组Ad-GFP组(P<0.05)。结论 Ad-SPK1可促进实验犬胃溃疡黏膜上皮修复和溃疡面血管增生,加快溃疡愈合。Objective To study the therapeutic effect of glycerine anti-itching lotion,minocycline in combination with nursing intervention on severe skin rashes induced by epidermal growth factor receptor tyrosine kinase inhibitors（EGFR-TKIs） in patients with non-small cell lung cancer（NSCLC）.Methods Grade 3 skin rashes induced by erlotinib or gefitinib were treated with oral minocycline（200mg,twice a day） and local application of glycerine anti-itching lotion（3 times a day） in combination with nursing intervention in 28 NSCLC patients.Results Skin rashes were completely disappeared in 10 patients,decreased to grade 1 in 14 patients and to grade 2 in 3 patients,progressed in 1 patient who withdrew from EGFR-TKIs treatment due to exacerbated subjective symptoms.The total effective rate was 96.4%.The HAMA and HAMD scores were significantly higher after intervention than before intervention（P0.001）.Conclusion Local application of glycerine anti-itching lotion and oral minocycline in combination with nursing intervention are effective against severe skin rashes induced by EGFR-TKIs.

关 键 词：胃溃疡 胃镜 鞘氨醇激酶 基因治疗 愈合 

分 类 号：R573.1[医药卫生—消化系统]