肝门部胆管癌裸鼠动物模型的建立  

Establishment of human hilar cholangiocarcinoma model in nude mice

作　　者：李成刚[1] 李崇辉[1] 张爱群[1] 董家鸿[1] 

机构地区：[1]解放军总医院肝胆外科研究所,北京100853

出　　处：《军医进修学院学报》2011年第5期511-512,518,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的建立胆管癌细胞系裸鼠肝门部胆管原位种植瘤模型。方法应用自制显微注射针将培养的胆管癌细胞系QBC939细胞接种于裸鼠肝门部胆管与门静脉组织间隙紧贴胆管处,15d后行种植瘤组织解剖学和病理学检查。结果每只裸鼠肝门部胆管与门静脉组织间隙内可接种浓度为1×107个/ml的胆管癌细胞悬液100μl;原位种植15d后,肝门部胆管原位种植瘤成瘤率为100%(7/7);病理学观察显示为典型的瘤组织表现。结论成功建立了胆管癌细胞系裸鼠肝门部胆管原位种植瘤模型。Objective To establish the implantation model of human in situ hilar cholangiocarcinoma cell line in nude mice.Methods Cultured human hilar cholangiocarcinoma cell line QBC939 was injected into the space between the hilar bile duct and portal vein of nude mice with a micro-injector.Anatomical and pathological features of the mice were detected 15d after the implantation model of human in situ hilar cholangiocarcinoma cell line was established.Results Suspension of 100ml of hilar cholangiocarcinoma cells at the concentration of 1×106/ml was injected into the space between the hilar bile duct and portal vein of each nude mouse.The successful rate was 100% 15d after the implantation model of human in situ hilar cholangiocarcinoma cell line.Histological examination showed typical tumor tissues in mice.Conclusion An implantation model of human in situ hilar cholangiocarcinoma can be established in nude mice.

关 键 词：胆管肿瘤 模型 动物 小鼠 裸 

分 类 号：R735[医药卫生—肿瘤]