人白血病相关蛋白16基因对3T3-L1脂肪细胞葡萄糖摄取及过氧化物酶体增殖物激活受体γ活性的影响  

Effects of LRP16 gene on glucose uptake and PPAR7 activity in 3T3-L1 adipocytes

作　　者：臧丽[1] 母义明[1] 吕朝晖[1] 汪保安[1] 窦京涛[1] 陆菊明[1] 潘长玉[1] 

机构地区：[1]解放军总医院内分泌科,北京100853

出　　处：《中国糖尿病杂志》2011年第4期293-297,共5页Chinese Journal of Diabetes

基　　金：973重大基础研究项目(2006CB503903);中华医学会临床医学科研专项基金(10020050227)

摘　　要：目的探讨人白血病相关蛋白(LRP)16对3T3-L1脂肪细胞葡萄糖摄取及过氧化物酶体增殖物激活受体(PPAR)γ活性的影响。方法利用脂质体转染及慢病毒介导的RNA干扰技术构建LRP16过表达、抑制表达及对照细胞系。检测LRP16对细胞葡萄糖摄取的影响。Luciferase法检测LRP16对PPAR反应元件(PPRE)相对荧光素酶活性的影响。Western Blot法检测LRP16对PPARγ及葡萄糖转运蛋白(GluT)-4表达的影响。结果 (1)成功构建LRP16过表达、抑制表达及对照细胞系。(2)过表达LRP1 6抑制细胞胰岛素刺激的葡萄糖摄取;抑制表达LRP16促进细胞胰岛素刺激的葡萄糖摄取。(3)LRP16剂量依赖性的抑制COS7细胞PPRE的相对荧光素酶活性;(4)LRP16抑制脂肪细胞PPARγ及GluT-4蛋白表达。结论 LRP16通过下调PPARγ活性抑制3T3-L1脂肪细胞葡萄糖摄取导致胰岛素抵抗。Objective To investigate the effect of LRP16 （leukemia related protein 16） gene on glucose uptake and PPARγ activity in 3T3-L1 adipocytes. Methods lipidosome transfection and lentivirus mediated siRNA technology were used to construct the cell lines. 2-deoxy-[3H]-D-glucose was used to measure glucose uptake. Luciferase was used to measure the relative luciferase activities of PPRE （PPAR-response elements） and Western blot was used to measure the expression of PPAR）, and GluT4 protein. Results （1） Cell line was successfully established. （2） Over-expression of LRP16 gene inhibited the glucose uptake, and down-expression of LRP16 sitimulated the glucose uptake when stimulated by insulin. （3） LRP16 gene inhibited the relative luciferase activity of PPRE in a dose dependent manner. （4） LRP16 gene inhibited the expression of PPARγ and GluT-4 protein. Conclusions LRP16 gene inhibits the glucose uptake and leads to insulin resistance through inhibiting the activity of PPARγ in 3T3-L1 adipocytes.

关 键 词：人白血病相关蛋白16 PPAR反应元件 过氧化物酶体增殖物激活体受体 葡萄糖摄取 3T3-L1脂肪细胞 

分 类 号：R733.7[医药卫生—肿瘤]