原发性胆汁性肝硬化患者外周血单个核细胞中趋化因子及其受体CCR6 mRNA的表达及其临床意义  

Increased expression of chemokine and CCR6 mRNA in peripheral blood mononuclear cells in patients with primary biliary cirrhosis and its clinical significance

作　　者：何铭珺[1] 杨再兴[1] 李畅[1] 陆慧琪[1] 叶伟民[1] 韩焕兴[1] 仲人前[1] 

机构地区：[1]解放军第二军医大学长征医院实验诊断科全军临床免疫中心,上海200003

出　　处：《临床军医杂志》2011年第2期224-227,共4页Clinical Journal of Medical Officers

基　　金：国家高科技研究发展计划(863计划)(2006AA02Z496);上海市科学技术委员会优秀学科带头人基金(07XD14013)资助

摘　　要：目的探讨原发性胆汁性肝硬化(PBC)患者外周血单个核细胞(PBMCs)中CC型趋化因子配体20(CCL20)和CC型趋化因子受体6(CCR6)mRNA的表达及临床意义。方法采用TaqMan荧光探针技术,建立实时荧光定量PCR的方法,分别检测50例PBC患者、50例乙型肝炎肝硬化患者及50例健康对照者PBMCs中CCL20和CCR6 mRNA的表达水平。结果 PBC患者中CCL20和CCR6的mRNA表达显著高于健康对照组和疾病对照组(P<0.01);并根据临床不同分期检测后发现,PBC患者中Ⅲ、Ⅳ期CCL20和CCR6 mRNA的表达较Ⅰ、Ⅱ期明显升高(P<0.05)。结论 PBC患者CCL20和CCR6 mRNA的表达与PBC的发生发展存在一定的相关性,可能参与PBC的调控机制,从而可能为PBC的诊断和预防提供一定的线索。Objective To explore expression of chemokine CCL20 and CCR6 mRNA in peripheral blood mononuclear cells in patients with primary biliary cirrhosis and its clinical significance.Methods A TaqMan probe technique and real-time PCR was used to measure the gene transcription levels of chemokine CCL20 and CCR6 mRNA in the Peripheral blood mononuclear cells（PBMCs） of 50 patients with PBC,50 patients with hepatitis B-related cirrhosis and 50 normal controls.Results The gene expression levels CCL20 and CCR6 in PBC group was significantly higher than that in healthy controls and hepatitis B related liver cirrhosis group（P0.01）.The mRNA expression levels of CCL20 and CCR6 were significantly higher in stage Ⅲ and Ⅳ patients compared with stageⅠ and Ⅱ patients.Conclusion A real-time quantitative RT-PCR method for detecting the expression of CCL20 and CCR6 gene in PBMC has been successfully established,and their expression levels were found to be correlated with the occurrence and development of PBC.Thus,CCL20 and CCR6 may be involved in the regulatory mechanisms associated with the development of PBC,and may be valuable in its diagnosis and prevention.

关 键 词：原发性胆汁性肝硬化 CC型趋化因子配体20 CC型趋化因子受体6 荧光定量PCR 

分 类 号：R575.22[医药卫生—消化系统]