COX-2在上皮性卵巢肿瘤的表达及塞来昔布对卵巢癌A2780细胞株凋亡的影响  

Expression of COX-2 in primary ovarian carcinoma and the effects of celecoxib on the cell apotosis of the human ovarian carcinoma cell line A2780

作　　者：徐晓辉[1] 申爱荣[1] 

机构地区：[1]郑州大学第三附属医院妇产科,河南郑州450052

出　　处：《中国肿瘤外科杂志》2011年第2期102-105,共4页Chinese Journal of Surgical Oncology

摘　　要：目的探讨环氧化酶-2(cyclooxygenase-2,COX-2)在上皮性卵巢肿瘤的表达及环氧化酶-2抑制剂塞来昔布(celecoxib)在体外对人卵巢癌细胞株A2780凋亡的影响。方法 (1)采用免疫组化SP法检测COX-2在65例上皮性卵巢癌,18例交界性、18例良性上皮性卵巢肿瘤,以及9例正常卵巢组织中的表达。(2)用流式细胞仪测定塞来昔布在体外对A2780细胞凋亡的影响。结果 (1)COX-2在上皮性卵巢癌组(73.8%)及交界性卵巢肿瘤组的表达(66.7%)显著高于良性卵巢肿瘤组(16.7%)及正常卵巢组(11.1%),P均<0.05。(2)流式细胞仪测定结果显示,塞来昔布呈剂量依赖方式诱导人卵巢癌A2780细胞凋亡(P<0.05)。结论 (1)COX-2在交界性及恶性上皮性卵巢肿瘤中的表达明显增强,COX-2可能在交界性及恶性上皮性卵巢肿瘤的发生发展中起一定作用。(2)体外特异性COX-2抑制剂塞来昔布能够诱导人卵巢癌A2780细胞株凋亡,可能成为上皮性卵巢癌化疗的有效药物。Objective To investigate the expression of cyclooxygenase-2 in epithelial ovarian carcinoma tissues and to investigate the effects of celecoxib on the cell apotosis of the human ovarian carcinoma cell line A2780. Methods （ 1 ） The immunohistochemistry was used to determine the expression of COX-2 in 65 primary ovarian carcinoma, 18 low malignant potential tumors in borderline, 18 cystadenomas and 9 normal ovaries. （2） Detect the effects of celecoxib on FCM, apotosis of ovarian carcinoma cells and its mechanism. Results （1） The COX-2 expression in epithelial ovarian cancer tissues and in borderline epithelial ovarian tumor tissUes were remarkably higher than that in benign epithelial ovarian tumor tissues and normal ovarian tissues（P 〈 0. 05 ）. （2） FCM showed celecoxib could cause apotosis of A2780 in a dosage dependent manner（ P 〈 0.05）. Conclusions （ 1 ） The COX-2 expression was higher in the tissue of malignant and borderline epithelial ovarian tumor. （2） In vitro, celecoxib could induce the apotosis of human ovarian adenocarcinoma cell line A2780. The COX-2 inhibitor may be a novel which is useful cbemoprevention agents for human ovarian

关 键 词：上皮性卵巢肿瘤 环氧化酶-2 塞来昔布 卵巢癌细胞株A2780 细胞凋亡 免疫组化 

分 类 号：R737.31[医药卫生—肿瘤]