猪宰后肌肉非乳酸供能特点及其与肉质性状相关性  

Characteristics of ATP-CP System Status in Postmortem Muscle and Their Associations with Pork Quality Traits

作　　者：门小明[1,2] 邓波[2] 徐子伟[1,2] 刘敏华[2] 齐珂珂[2] 

机构地区：[1]江南大学食品科技学院,江苏无锡214122 [2]浙江省农业科学院畜牧兽医研究所,杭州310021

出　　处：《中国农业科学》2011年第7期1457-1465,共9页Scientia Agricultura Sinica

基　　金：浙江省重大科技专项项目(2008C02003-1);国家生猪产业技术体系项目

摘　　要：【目的】揭示猪宰后肌肉的非乳酸(CP+ADP→Cr+ATP,ATP-CP)供能特点及其与肉质性状的关系,为猪肉品质调控与形成机制的研究提供参考。【方法】选取上市体重金华猪、浙江中白猪、杜浙猪(杜洛克×浙江中白猪)和杜大长猪(杜洛克×(大约克×长白猪))阉公猪各8头,屠宰后测定背最长肌和腰大肌的肉质性状(pH45min pH24h、剪切力(SF)、系水力(WHC)、宰后24h蛋白质溶解度)、肌酸激酶(creatine kinase,CK)与三磷酸腺苷酶(adenosinetri phosphatase,ATPase)活性、高能磷酸化合物(adenosine diphosphate,ADP;adenosine triphosphate,ATP;creatine,cr;creatine phosphate,CP)含量,并用M=([ATP]+[Cr])/([ADP]+[CP])表示宰后45min的ATP-CP反应进程,用N=(CK+ATPase)×m表示ATP-CP供能潜力,比较各指标的品种或部位间差异,分析ATP-CP状态与肉质性状的关系。【结果】与背最长肌相比,腰大肌表现较高的CK活性、PH24h、肌肉总蛋白溶解度(杜浙猪除外)、肌纤维蛋白溶解度、M值和N值(金华猪除外)(P<0.05),表现较低的pH45min(金华猪除外)、△pH、剪切力(杜大长猪除外)、系水力、肌浆蛋白溶解度(P<0.05);两部位肌肉的CK活性、ATPase活性和背最长肌的M值和N值按照金华猪→浙江中白猪→杜浙猪→杜大长猪的顺序表现降低趋势,腰大肌的M值和N值表现相反,而两部位肌肉pH45min和△pH均按此顺序表现升高趋势;背最长肌的M值与pH45min、△pH呈负相关(r=-0.811,r=-0.908,P<0.01),N值与pH24h呈正相关(r=0.876,P<0.01),与△pH、pH45min呈负相关(r=-0.711,P<0.01;r=-0.385,P<0.05),与其它肉质性状的相关性表现复杂。【结论】宰后肌肉ATP-CP反应进程(M)和反应潜力(N)在背最长肌随本地猪血统比例增加而增大,在腰大肌随本地猪血统比例增加而降低,在金华猪腰大肌低于背最长肌,在其它品种腰大肌高于背最长肌,并与宰后肌肉pH密切相关,可以减缓宰后24h内pH下降程度,但与其它肉质性状的关系需�【Objective】To study the role of ATP-CP system in postmortem muscle on the formation of pork quality, this research investigated the effects of pig breeds and muscle types on ATP-CP status and their possible associations with pork quality traits. 【Method】Longissimus dorsi （L.D.） and psoas major （P.M.） of Jinhua pure breed （JH）, Zhongbai pure breed （ZB）, Duroc ×Zhongbai （DZ） cross and Duroc × （Yorkshire×Landrace） （DYL） cross were sampled, and ATP-CP status （including the concentration of ADP, ATP, creatine and creatine phosphate and creatine kinase, ATPase activities） and meat quality traits （including pH45min, pH24h, shear force （SF）, water-holding capacity （WHC）, total protein solubility （TPS）, sarcoplasmic protein solubility （SPS） and myofibril protein solubility （MPS）） were measured. The reaction process and the potentiality of ATP-CP pathway were described as M=（[ATP]＋[Cr]）/（[ADP]＋[CP]） and N=（CK＋ATPase）× M, respectively. One-way ANOVA and Duncan＇s test were employed to analyze the difference among different pig breeds. Independent T- test was used to analyze the difference between L.D. and P.M. 【Result】 P.M. exhibited a higher CK activity, pH24h, TPS, MPS, M-value, N-value （P〈0.05） and a lower pH45min, △pH, SF, WHC, SPS than L.D. （P〈0.05）, the activities of CK and ATPase activities in both L.D. and P.M. muscles, M-value and N-value in L.D. muscle but not P.M. muscle declined according to the order of JH→ZB→DZ→DYL, pH45min and △pH in both muscles increased according to the above order. A negative correlation between M-value and pH45min （r=-0.811,P〈0.01） or △pH （r=-0.908, P〈0.01） or between N-value and △pH （r=-0.711, P〈0.01） or pH45min （r=-0.385, P〈0.05）, a positive correlation between N-value and pH24h （r=0.876, P〈0.01） were found in L.D. muscle. 【Conclusion】 The results indicated that ATP-CP reaction process （M） and potentiality （N） were both increased

关 键 词：非乳酸供能 猪肉品质 肌肉部位 

分 类 号：S828[农业科学—畜牧学]