槲寄生提取物抗缺血性心律失常作用的研究  

Pharmacological effect of viscum coloratum extract on ischemic arrhythmia

作　　者：何秀权[1] 栾海蓉[1] 刘清梅[1] 侯云龙[1] 吕延杰[1] 

机构地区：[1]哈尔滨医科大学药理学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2010年第6期519-523,共5页Journal of Harbin Medical University

基　　金：黑龙江省科学技术厅重点项目(DC06C33302)

摘　　要：目的研究槲寄生提取物(viscum coloratum extract,VCE)抗缺血性心律失常的药理作用。方法采用大鼠冠脉结扎的方法建立心肌梗死模型,观察VCE对心肌梗死大鼠心律失常发生率和心肌梗死面积的影响。制备大鼠离体右心房标本,在浴槽中观察VCE对大鼠右心房收缩性的影响。结果注射VCE各组与对照组比,心律失常发生减少,心肌梗死面积缩小。低剂量VCE可使大鼠离体右心房收缩力呈现正性肌力,而高剂量VCE呈现负性肌力。VCE的负性肌力作用可被阿托品阻断。高剂量VCE可使大鼠离体右心房收缩频率降低;VCE能使异丙肾上腺素对心肌的收缩作用降低;使CaCl2量效曲线下移。结论 VCE有抗缺血性心律失常的作用,高剂量VCE能使大鼠离体右心房表现为负性肌力和负性频率作用,还能对抗异丙肾上腺素和CaCl2的正性作用,VCE的负性肌力作用可被阿托品阻断提示其抗缺血性心律失常作用可能与激动M受体或其某一亚型有关。Objective To study the pharmacological effect of the viscum coloratum extract（VCE） against ischemic arrhythmia.Methods To establish the myocardial ischemia model,left anterior descending coronary artery of rats was ligated.The incidence of cardiac arrhythmia and the size of myocardial infarction were investigated.The right atrium of rats was isolated and incubated in the bath to observe the effect of VCE.Results The incidence of arrhythmias and myocardial infarction size in VCE groups were reduced significantly.VCE-induced contraction was increased in the isolated right atrium of rats at low-dose of VCE whereas the opposite responses were confirmed at high-dose of VCE.The effect of VCE on isolated right atrium of rats was blocked by AT.The frequency of isolated right atrium contraction was reduced in VCE group compared with that in the control.Compared with the control,pre-incubation with VCE not only reduced the contraction but also the frequency in the presence of isoprenaline.Additionally,pre-incubation of VCE also shifted CaCl2 dose-effect curve to the right.Conclusion VCE possessed anti-arrhythmias induced by myocardial ischemia.The underlying mechanisms may be related to its negative inotropics,negative chronotropics,and M-receptor activation.

关 键 词：槲寄生提取物 缺血性心律失常 心肌收缩力 M受体 

分 类 号：R-332[医药卫生] R284.2