乳腺癌中RUNX3基因启动子区甲基化及基因表达研究  

Research on promoter methylation and transcriptional expression of RUNX3 gene in breast cancers

作　　者：娄阁[1] 姜影[1] 杜金荣[1] 

机构地区：[1]哈尔滨医科大学第二临床医学院病理科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2010年第6期567-571,共5页Journal of Harbin Medical University

摘　　要：目的通过检测乳腺癌中RUNX3基因启动子甲基化状态及其对mRNA表达的影响,探讨RUNX3在乳腺癌发生、发展中的意义。方法运用甲基化特异性PCR(MSP)检测40例乳腺浸润性导管癌和20例乳腺良性病变标本中RUNX3基因启动子甲基化状态,同时采用逆转录聚合酶链反应(RT-PCR)检测该基因的表达情况。结果乳腺癌组织中RUNX3基因的甲基化率为47.5%,明显高于乳腺良性病变组织的甲基化率,其差异具有显著性(P<0.05);同时乳腺癌组织中RUNX3基因表达缺失率为40%,明显高于乳腺良性病变,其差异也具有显著性(P<0.05)。乳腺癌中RUNX3基因启动子甲基化水平与患者的病理组织学分级有相关性,差异有统计学意义(P<0.05),与患者的年龄、淋巴结转移无相关性。RUNX3基因表达与患者的临床病理特征均无相关性。结论 RUNX3基因启动子的高甲基化可能是乳腺癌中一类重要的分子改变,也是导致RUNX3基因表达下降的原因之一,并参与了肿瘤的演进。Objective To investigate the promoter methylation status and expression of tumor suppressor gene RUNX3,and their roles in the carcinogenesis and development in invasive ductal carcinoma.Methods MSP（methylation specific PCR） was used to measure the promoter methylation status of 40 cases of breast invasive ductal carcinoma and 20 cases of benign breast lesions.RUNX3 mRNA expression level was measured by reverse transcription-polymerase chain reaction（RT-PCR）.Results The frequency of promoter methylation of RUNX3 gene in breast cancer was 47.5%,which was higher significantly than that of benign breast lesion（P0.05）.The loss frequency of RUNX3 mRNA expression was 40.0% in breast cancers,which was higher significantly than that of benign breast lesion（P0.05）.The methylation of RUNX3 promoter in breast invasive ductal carcinoma was not correlated with the age of patient and lymph node metatasis,but correlated with the histological grading（P0.05）.The mRNA expression level of RUNX3 was not correlated with all the clinical pathological characters.Conclusion The promoter methylation of RUNX3 may be an important molecular change and may contribute to the lowlevel of RUNX3 mRNA expression in breast invasive ductal carcinoma,and maybe play a certain role in malignant progression.

关 键 词：乳腺癌 RUNX3基因 甲基化 RT-PCR 

分 类 号：R737.9[医药卫生—肿瘤]