临床膀胱癌组织中miRNA的差异表达研究  

Differentially expressed miRNA in bladder cancer

作　　者：夏伟[1] 宋涛[2] 李洁[1] 黄宝春[1] 李少华[1] 丁红梅[1] 王天有[3] 邵宁生[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]解放军总医院泌尿外科,北京100853 [3]首都儿科研究所附属儿童医院,北京100020

出　　处：《军事医学科学院院刊》2010年第6期540-542,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：国家重点基础研究发展计划(973计划)(2005CB724600);国家自然科学基金(30600110;30971630);国家高技术研究发展计划(863计划)(2007AA021004)

摘　　要：目的寻找并鉴定在膀胱癌及癌旁组织中差异表达的微RNA(miRNA)。方法结合miRNA芯片技术寻找膀胱癌及癌旁组织中差异表达的miRNA,并通过荧光定量PCR进行验证。结果在癌与癌旁组织中共检测到115条存在显著性差异的miRNA,其中膀胱癌组织中下调42条,上调73条。结论膀胱癌组织与癌旁组织中miRNA表达谱存在较大差异,miRNA与膀胱癌的发生发展存在一定的相关性。Objective To identify the differentially expressed microRNA（miRNA） in bladder cancer.Methods The miRNA was detected by miRNA array and examined by qRT-PCR.Results A total of 115 differentially expressed miRNAs were detected by miRNA array in clinical bladder cancer and paracancer tissues,42 of which were low-expressed while 73 of them were highly-expressed in bladder cancer tissues.Conclusion The miRNA expression profile is different in bladder cancer and paracancer tissues,suggesting that miRNA might be involved in the initiation and development of bladder cancer.

关 键 词：膀胱肿瘤 微RNA 聚合酶链反应 基因 肿瘤抑制 

分 类 号：R737.14[医药卫生—肿瘤]