甘薯提取物有效成分鉴定及其对破骨细胞形成分化的影响  

The identification of the effective component in sweet potato extracts and its effect on the formation and differentiation of osteoclast

作　　者：吴小玉[1,2] 唐全勇[1] 屈鹏飞[1] 陈彪[1] 杨威[1] 仝金秋[3,4] 张英怀[1] 

机构地区：[1]河北医科大学第二医院口腔颌面外科,石家庄市050000 [2]河北正定县医院口腔科 [3]河北医科大学第二医院手术室,石家庄市050000 [4]河北省正定县医院手术室

出　　处：《河北医药》2011年第7期985-987,共3页Hebei Medical Journal

摘　　要：目的对甘薯提取物有效化学成份进行鉴定,并观察各有效成分对破骨细胞形成分化的影响。方法采用高效液相色谱对甘薯提取物有效成份进行鉴定,同时采用3种不同细胞培养系,观察甘薯提取物4种有效成分对破骨细胞形成分化的影响。结果甘薯提取物有效成分包括咖啡酸、绿原酸、异构绿原酸和奎宁酸。在4种有效成分中,咖啡酸对破骨细胞形成分化的影响最大,直接抑制破骨细胞的形成和分化,而奎宁酸未显示出有效的抑制作用。结论甘薯提取物4种有效成分对破骨细胞形成分化均有影响,其中咖啡酸影响最大,对破骨细胞形成分化的抑制作用最强。Objective To identify effective component in sweet potato extract,and to observe the effect of the effective components on the formation and differentiation of osteoclast.Methods The effective components in sweet potato extracts were identified by using high performance liquid chromatography （HPLC）.The effects of four kinds of effective components in sweet potato extracts on the formation and differentiation of osteoclast were observed by using three different cell culture systems.Results The effective components in sweet potato extracts included coffeic acid,chlorogenic acid,iso-chlorogenic acid and quinic acid.Among the four kinds of effective components,the effect of coffeic acid was most remarkable on the formation and differentiation of osteoclast.The coffeic acid inhibited directly the formation and differentiation of osteoclast,however,the inhibition effect of quinic acid was not obvious.Conclusion The four kinds of effective components in sweet potato extracts can affect the formation and differentiation of osteoclast,and the effect of coffeic acid is the most obvious and its inhibition effect on the formation and differentiation of osteoclast is the strongest.

关 键 词：甘薯提取物 高效液相色谱 破骨细胞 细胞培养 

分 类 号：R782.2[医药卫生—口腔医学]