Identification and Purification of an Allergic Glycoprotein from Ginkgo biloba Kernel  

作　　者：YANG Jian-ting WU Cai-e LI Ying-ying JIA Shao-qian FAN Gong-jian PENG Fang-ren 

机构地区：[1]Department of Forest Resource and Environment, Nanjing Forest University, Nanjing 210037, P.R.China [2]College of Food and Drug, Anhui Science and Technology University, Fengyang 233100, P.R. China

出　　处：《Agricultural Sciences in China》2011年第4期631-641,共11页中国农业科学（英文版）

基　　金：supported by Research Fund for the Doctoral Program of Higher Education of China(200802980004 and 20070298008);the National Natural Science Fundation of China (30872055)

摘　　要：Glycoprotein from Ginkgo biloba kernel may be allergic. In this paper, the allergic proteins were identified with Western blotting, and the 32 kDa glycoprotein was purified with ion exchange chromatography and gel chromatography. With Western blotting, there were 3 allergic proteins with molecular weight 21, 32, and 36 kDa. With SDS-PAGE analysis and measurements of the protein and sugar contents, the isolation and purification technology of 32 kDa was confirmed. Ginkgo crude protein extract was precipitated with ammonium sulphate （saturation gradient： 40-80%）. The precipitate was purified by chromatography with DEAE-cellulose 52, then chromatography with Sephadex G-200 and the target glycoprotein was finally obtained. The analysis results showed the molecule of the glycoprotein was 32.12 kDa and the ratio of protein to sugar was 20.56：1. In conclusion, the purification method could be used in preparation of the glycoprotein, and the study could provide a basis for the further research of the glycoprotein.Glycoprotein from Ginkgo biloba kernel may be allergic. In this paper, the allergic proteins were identified with Western blotting, and the 32 kDa glycoprotein was purified with ion exchange chromatography and gel chromatography. With Western blotting, there were 3 allergic proteins with molecular weight 21, 32, and 36 kDa. With SDS-PAGE analysis and measurements of the protein and sugar contents, the isolation and purification technology of 32 kDa was confirmed. Ginkgo crude protein extract was precipitated with ammonium sulphate （saturation gradient： 40-80%）. The precipitate was purified by chromatography with DEAE-cellulose 52, then chromatography with Sephadex G-200 and the target glycoprotein was finally obtained. The analysis results showed the molecule of the glycoprotein was 32.12 kDa and the ratio of protein to sugar was 20.56：1. In conclusion, the purification method could be used in preparation of the glycoprotein, and the study could provide a basis for the further research of the glycoprotein.

关 键 词：Ginkgo biloba kernel GLYCOPROTEIN ALLERGY IDENTIFICATION PURIFICATION 

分 类 号：Q513.2[生物学—生物化学] O636.1[理学—高分子化学]