慢性粒细胞白血病和真性红细胞增多症bcr/abl融合基因表达及临床意义  

Expression of Bcr/abl Fusion Gene in Chronic Mye logenous Leukemia,Polycythemia Vera and Their Clinical Significance

作　　者：陈燕[1] 李惠玉[1] 吴裕丹[1] 

机构地区：[1]同济医科大学附属协和医院血液病研究所

出　　处：《临床血液学杂志》1999年第4期156-158,共3页Journal of Clinical Hematology

摘　　要：目的：探讨慢性粒细胞白血病和真性红细胞增多症ｂｃｒ／ａｂｌ融合基因出现的频率及临床意义。方法：采用逆转录多聚酶链反应（ＲＴ－ＰＣＲ）技术，研究了慢性粒细胞白血病（ＣＭＬ）、真性红细胞增多症（ＰＶ）ｂｃｒ／ａｂｌ基因的变化。结果：４８ 例慢性期ＣＭＬ，４３ 例呈现ｂｃｒ／ａｂｌ基因阳性，占８９．６％ ，１４ 例加速，急变期ＣＭＬ，６ 例出现ｂｃｒ／ａｂｌ基因，阳性率为４２．９％ ，与慢性期ＣＭＬ相比Ｐ＜ ０．０１；１０ 例ＰＶ出现１ 例ｂｃｒ／ａｂｌ基因阳性，占１０．０％ 。结论：ｂｃｒ／ａｂｌ基因检测对于ＣＭＬ的临床分型有重要意义，ｂｃｒ／ａｂｌ阴性的ＣＭＬ预后差，而ｂｃｒ／ａｂｌ基因的阳性有助于初诊时的ＣＭＬ急变与急性非淋巴细胞白血病（ＡＭＬ）Ｍ２ 的鉴别和ＣＭＬ与慢性粒单细胞白血病（ＣＭＭＬ）的鉴别。Objective:To study on frequencies of bcr/abl fusion gene in chronic myelogenous leukemia,polycythemia vera and their clinical significance.Method:Using RT PCR technique,the change of bcr/abl genes in chronic myelogenous leukemia (CML) and polycythemia vera (PV) were investigated.Result:43 cases showed positive bcr/abl gene out of 48 cases of chronic phase with CML.It accounted for 90%.6 cases showed positive bcr/abl gene out of 14 cases of accelerated phase and blast crisis of CML.It accounted for 43%.Compared with chronic phase,there was statistical difference.(P< 0.01 )1 case showed positive bcr/abl gene out of 10 cases with PV.It accounted for 10%.Conclusion:There was an important clinical typing significance in CML using bcr/abl gene detecting technique.The prognosis showed worse in patients of negative bcr/abl gene.When bcr/abl gene showed positive,it contributed to differential diagnosis in blast crisis of CML and acute myelogenous leukemia (AML) M2 subtype at the first diagnosis,and in CML and chronic myelomonocytic leukemia (CMML).

关 键 词：白血病 慢性 真性红细胞增多 BCR/ABL基因 

分 类 号：R733.72[医药卫生—肿瘤]