吸毒合并HIV感染与HLA-DRB1*1301/1302等位基因的相关性  

Relation between HLA-DRB1*1301/1302 Allele Polymorphism and HIV Infection in Drug Users

作　　者：刁波[1] 刘莹[2] 申元英[3] 周芳[1] 冯勇[4] 罗凡[4] 侯炜[4] 

机构地区：[1]广州军区武汉总医院医学实验科,湖北武汉430070 [2]广州军区武汉总医院检验科,湖北武汉430070 [3]大理学院公共卫生学院 [4]武汉大学医学病毒学研究所病毒学国家重点实验室

出　　处：《华南国防医学杂志》2011年第2期113-115,共3页Military Medical Journal of South China

基　　金：国家自然科学基金支持项目(30972754)

摘　　要：目的探讨吸毒并HIV感染与HLA-DRB1*1301/1302等位基因的相关性,为寻找吸毒合并HIV感染的易感基因和抵抗基因提供线索。方法应用序列特异性引物聚合酶链反应(polymerase chain reaction with sequence-specific primers,PCR-SSP)技术对41例无亲缘关系的吸毒合并HIV感染者及24例单纯吸毒者进行HLA-DRB1*1301/1302等位基因进行检测,分析正常对照组和吸毒组及吸毒合并HIV感染组HLA-DRB1 1301/1302等位基因频率分布。结果正常对照组*1301/1302等位基因频率为0.06573。吸毒组等位基因频率为0.04167,RR=0.600649,与正常组比较有下降趋势,但无统计学意义(P>0.05);吸毒合并HIV感染组等位基因频率为0.01220,RR=0.1700,与正常组比较有显著下降趋势(P<0.05)。吸毒组与吸毒合并HIV感染组等位基因频率无统计学差异(P>0.05)。结论 HLA-DRB1*1301/1302等位基因动态性与吸毒合并HIV感染的抵抗性密切相关,这对阐明疾病的病理、治疗和免疫预防具有重要作用。Objective To investigate the relationship between HLA-DRB1＊1301/1302 allele polymorphism and HIV infection in drug users,and provide a cue for searching the susceptible allele and resistant allele.Methods A total of 41 drug users with HIV infection and 24 drug users without genetic relationship were studied.HLA-DRB1＊1301/1302 allele polymorphism were tested by polymerase chain reaction with sequence-specific primers（PCR-SSP）,and its frequency distribution were analyzed and compared between two groups.Results The gene frequency of ＊1301/1302 allele in the control group was 0.06573.In drug user group,the frequency was 0.04167,RR=0.600649,and decreased but not significantly compared with control group（P0.05）.In HIV infected drug users,the frequency was 0.01220,RR=0.1700,and decreased significantly compared with control group（P0.05）.Compared with drug users group,there was no significant difference in the frequency between drug users and HIV infected drug users（P0.05）.Conclusion There exists close relationship between HLA-DRB1＊1301/1302 alleles and resistance of drug use with HIV infection,which plays a crucial role in the pathology,treatment and immune prevention of the disease.

关 键 词：HLA-DRB1 等位基因 吸毒合并HIV感染 

分 类 号：R512.91[医药卫生—内科学]