改良法培养人牙髓间充质干细胞及其向神经样细胞的分化  

Differentiation of modified method cultured human dental pulp mesenchymal stem cells into neuron-like cells

作　　者：李远[1,2] 关方霞[3] 李季[4] 杨波[1,2] 胡祥[5,6] 杜英[7] 谷晨熙[8] 雷宁静[3] 王晓薇[9] 

机构地区：[1]郑州大学第一附属医院神经外科,河南省郑州市450052 [2]河南省高等学校临床医学重点学科开放实验室,河南省郑州市450052 [3]郑州大学生物工程系,河南省郑州市450001 [4]郑州大学第一附属医院口腔科,河南省郑州市450052 [5]江苏省干细胞与生物治疗公共技术服务平台,江苏省泰州市225300 [6]深圳市北科细胞工程研究所,广东省深圳市518000 [7]郑州大学基础医学院微生物与免疫学教研室,河南省郑州市450052 [8]郑州大学临床医学系,河南省郑州市450001 [9]湖南师范大学生命科学学院,湖南省长沙市410081

出　　处：《中国组织工程研究与临床康复》2010年第49期9239-9243,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：郑州大学211三期建设项目"干细胞基础与临床研究";江苏省干细胞与生物治疗公共技术服务平台(BM2008146)~~

摘　　要：背景:应用改良组织原位培养法后,牙髓间充质干细胞的提取成功率显著提高,但鲜有文献报道其与酶消化法提取干细胞生物学特性的异同。目的:观察改良组织原位培养法与传统酶消化法所获得的牙髓间充质干细胞向神经样细胞分化上的差异。方法:将同一组织来源的标本随机分成两组,分别用改良的组织原位培养法及常规的酶消化法提取人牙髓间充质干细胞,通过免疫组化、流式细胞检测及RT-PCR等比较观察其生长情况、抗原表达及向神经样细胞分化能力。结果与结论:改良法原位培养组分离牙髓间充质干细胞与常规分离培养组分离的干细胞在形态学表现,抗原表达及神经向分化能力上无明显区别,但简化了操作流程,且所得细胞数量大。BACKGROUND：The success rate of dental pulp stem cells（DPSCs） which isolated by the modified cultured method of tissue is elevated.However,few reports have concerned the difference in biological features between DPSCs and stem cells extracted by emzyme digestion method.OBJECTIVE：To observe the difference in the differentiation of DPSCs isolated by the new method and isolated by traditional method into neuron-like cells.METHODS：The specimen of the same tissue was randomly divided into two parts：one is isolated by the modified cultured method of tissue（A group）,another is isolated by the traditional method（B group）.Growth,antigen expression and differentiation of DPSCs into neuron-like cells were observed and compared using immunohistochemical method,flow cytometry and reverse transcription-polymerase chain reaction.RESULTS AND CONCLUSION：There were no distinct changes in morphology expression,antigen ability,differentiation ability into neurons between A group and B group.However,A group had simple process and obtained a large quantity.

关 键 词：改良法 原位培养 干细胞 牙髓 神经分化 

分 类 号：R394.2[医药卫生—医学遗传学]