Genistein对APP695基因转染PCI2细胞Caspase-3表达的影响  

The Effect of Genistein on the Expression of Caspase-3 Protein in PCI 2 Cells Transfected with Mutant APP695 Type

作　　者：赵翠香[1] 龙云[2] 王凤斌[1] 张丽娟 

机构地区：[1]潍坊医学院生理学教研室,山东潍坊261053 [2]重庆三峡医药高等专科学校 [3]潍坊市坊子区人民医院脑科医院

出　　处：《潍坊医学院学报》2010年第6期456-459,共4页Acta Academiae Medicinae Weifang

基　　金：山东省科技发展计划项目（课题编号：2007GG30002007）

摘　　要：目的利用APP695MT基因转染PCI2细胞为细胞模型，观察植物雌激素三羟基异黄酮（GST）对PCI2细胞中Caspase-3表达的影响，初步探讨GST对表达APP695MT基因PCI2细胞的保护作用及其相关机制。方法将培养的PCI2细胞分为正常对照组、APP695转染组、GST干预组，免疫细胞化学SABC法检测凋亡相关蛋白Caspase-3在不同处理组PCI2细胞中的表达变化。，结果正常纽及空载组Pc12细胞Caspase-3阳性表达非常弱；与正常对照组比较，APP695转染组PCI2细胞Caspase-3为强阳性表达（P〈0.01）；GST干预组与APP695转染组比较．Caspase-3的免疫反应产物明显减弱（P〈0．01），且15Ixmol／L和201xmol／LGST处理组与5txmol／L和10gmol／LGST处理组比较，Caspase-3的免疫反应产物减弱更明显（P〈0．01）。结论APP695基因转染的PCI2细胞中凋亡蛋白Caspase-3的表达明显增强，GST的干预能显著降低其表达，对神经细胞产生保护作用。Objective To investigate the protective effect of Genistein against apoptosis of PC12 cells induced by transfection mutant APP695 plasmid DNA and its impact on Caspase-3. Methods The AD cell model were established by transfecting the mutant APP695 plasmid DNA to PC12 cells,the expression of Caspase-3 protein in PC12 cells was obselwed by Imnumocytochemistry SABC. Results The expression level of activated Caspase-3 in normal control group and Unladen group was lower （ P 〉 0.05 ） ; Compared with the normal control group, the expression level of caspase-3 in APP695 transfected group was strongly increased（P 〈 0.01 ） ;compared with APP695 transfected group,the immune reaction of caspase-3 in all GST treatment groups were significantly decreased （ P 〈 0.01）. Conclusion In APP695MT transfected PCI 2 cells,the expression level of Caspase-3 was increased,while GST could reduce the level of Caspase-3 protein in the intervention groups,which confirmed that GST has protective effects on neurons.

关 键 词：GENISTEIN APP695 PCI 2细胞 CASPASE-3 免疫组化SABC 

分 类 号：R338[医药卫生—人体生理学]