碱性成纤维细胞生长因子对人牙髓细胞增殖和分化的作用  

The effects of basic fibroblast growth factor on proliferation and differentiation of human dental pulp cells in vitro

作　　者：郝建军[1] 汪平[1] 史俊南[1] 牛忠英[1] 肖明振[1] 王浩军[2] 

机构地区：[1]第四军医大学口腔医学院,西安710032 [2]第四军医大学,西安710032

出　　处：《中华口腔医学杂志》1999年第4期239-241,共3页Chinese Journal of Stomatology

基　　金：国家自然科学基金!资助课题 (3970 0 1 60 )

摘　　要：目的　探讨碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)对培养人牙髓细胞增殖和分化的效应。方法　采用四唑盐法、3HTdR掺入法、图象分析,检测重组人bFGF(hbFGF)对细胞DNA、胶原蛋白、纤维粘连蛋白、碱性磷酸酶、骨形成蛋白合成和凝集素表达的影响。结果　hbFGF浓度在1～10μg/L时显著促进细胞的增殖,浓度为1～100μg/L时可显著刺激人牙髓细胞DNA的合成。hbFGF在高浓度下(10～1000μg/L),Ⅰ型胶原、纤维粘连蛋白、刀豆凝集素A受体和碱性磷酸酶的表达增强,而Ⅲ型胶原和麦胚凝聚素受体的合成受到抑制,不同浓度的bFGF对细胞骨形成蛋白的表达无明显影响。Objective To evaluate the effects of human basic fibroblast growth factor (hbFGF) on proliferation and differentiation of human dental pulp cells in vitro.Methods The syntheses of DNA, collagen, fibronection (FN), alkaline phosphatase (ALP) and bone morphogenetic protein (BMP), and the expression of agglutinin were measured with imagine processing and analysis system. Results hbFGF at concentration of 1～10 μg/L stimulated the cell proliferation measured by MTT colorimetric assay, and promoted incorporation of 3H thymidine at 1～100μg/L. The expression of type Ⅰ collagen, FN, Con A receptor, and ALP significantly increased at hbFGF concentration of 10～1 000 μg/L, but the expression of type Ⅲ collagen and WGA receptor markedly decreased at the same concentration. There was no significant difference in expression of BMP.Conclusion bFGF has the capability to promote the differentiation of dental pulp cells to odontoblasts in culture.

关 键 词：细胞培养 BFGF 牙髓细胞增殖 细胞分化 

分 类 号：R781.302[医药卫生—口腔医学]