白血病粒单祖细胞的极限稀释微孔培养  

Limiting Dilution Microculture of Granulocyte-Macrophage Progenitor in Acute and Chronic Myelogeneous Leukemia

作　　者：黄士昂[1] 李崇渔[1] 王辨明 喻冬姣 

机构地区：[1]同济医科大学血液病研究所

出　　处：《同济医科大学学报》1990年第6期393-395,共3页Acta Universitatis Medicinae Tongji

摘　　要：本文将极限稀释微孔培养CFU-GM技术初步用于急性非淋巴细胞白血病(ANLL)和慢性粒细胞白血病(CML)研究中,对两者的CFU-GM进行了细胞形态学、细胞化学染色及生化标记、扫描电镜、~3H-TdR掺入和细胞遗传学等方面的研究,证实极限稀释微孔培养较琼脂半固体培养更有利于白血病克隆祖细胞的生长。In vitro granulocyte-macrophage progenitor (CFU-GM) with limiting dilution microculture was preliminarily used in the study of acute and chronic myelogeneous leukemia (AML and CML).It was proved that CFU-GM in AML and CML were generated more suitably in the limiting dilution microculture than in semisolid agarculture by the studies of CFU-GM with cytomorphology,cloning efficiency,cytochemistry,scanning electron microscopy,3H-TdR uptake and cytogenetics.In addition,four different growth patterns were observed in vitro.

关 键 词：白血病 粒单祖细胞 CFU-GM 

分 类 号：R733.7[医药卫生—肿瘤]