尾加压素Ⅱ对大鼠血管外膜阳离子氨基酸转运体的影响  

Effect of Urotendin Ⅱ on Cationic Amino Acid Transporter(CAT-1 and CAT-2B) in Isolated Aortic Adventitia of Rats

作　　者：闫琳[1] 梁颖 薛立华[2] 黎济荣[2] 贾月霞[1,2] 

机构地区：[1]宁夏医科大学基础医学院实验中心,银川750004 [2]宁夏医科大学基础医学院病理生理学系,银川750004

出　　处：《宁夏医科大学学报》2011年第3期227-230,共4页Journal of Ningxia Medical University

基　　金：宁夏医科大学基金(NZ0542)

摘　　要：目的观察尾加压素Ⅱ（UII）对大鼠主动脉血管外膜阳离子氨基酸转运体（CAT-1、CAT-2B）和诱导型一氧化氮合酶（iNOS）基因表达影响。方法 SD大鼠24只,250~300g,随机分为空白对照组、UII组（10-9和10-8mol.L-1）和脂多糖（LPS）阳性对照组,每组6只。分离雄性SD大鼠胸主动脉外膜,分别加入单纯孵育液、不同浓度UII和LPS孵育6h。测定孵育液中亚硝酸盐（NO2-）含量;RT-PCR方法测定CAT-1、CAT-2B和iNOS mRNA基因表达。结果与对照组比较UII（10-9~10-8.L-1）刺激血管外膜NO2-生成增加（27%,P〈0.05,和49%,P〈0.01）;UII组血管外膜阳离子氨基酸转运体（CAT-1和CAT-2B）mRNA水平增加（均P〈0.01）,iNOS mRNA表达增加（P〈0.01）。结论 UII可激活血管外膜CAT-1和CAT-2B基因表达,其变化可能参与了NO释放从而引起血管的扩张。Objective To assess the gene expression of cationic amino acid transporter-1（CAT-1）,CAT-2B and inducible nitric oxide synthase（iNOS） in urotensin II（UII）-stimulated rat vascular adventitia.Methods The aortic adventitia were incubated in vitro.24 rats were randomly divided into normal group,2U Ⅱ groups and LPS group.The aortic adventitia NO production was determined.Semi-quantitative RT-PCR was used to determine the mRNA concentrations of iNOS and CAT-1 and CAT-2B in aortic adventitia.Results After incubation of aorta for 6 hours,nitrite production in U Ⅱ group significantly increased（27%,P0.05,49%,P0.01）.UII-induced CAT-1、CAT-2B and iNOS mRNA increased significantly in aortic adventitia.Conclusion The results illustrate that U Ⅱ induces the transcription of high-affinity CAT-1 and CAT-2B in aortic adventitia.Transcription of CAT-1 and CAT-2B are correlated well with aortic NO biosynthesis.

关 键 词：尾加压素Ⅱ 一氧化氮 一氧化氮合酶 阳离子氨基酸转运体 

分 类 号：R363[医药卫生—病理学]