γ干扰素对肾小球肾炎大鼠肾组织Smad7和Ⅳ型胶原mRNA表达的影响  

Effects of IFN-γ on the expression of Smad7 and Col Ⅳ in renal tissue of rat with glomerulonephritis

作　　者：姜琳[1] 孙灿林[1] 肖蔚[2] 蒋小芹[2] 李慧[3] 于鸿[2] 

机构地区：[1]泰州市人民医院麻醉科,江苏泰州225300 [2]泰州市人民医院病理科,江苏泰州225300 [3]复旦大学上海医学院病理系,上海200032

出　　处：《江苏大学学报（医学版）》2011年第2期143-146,F0003,共5页Journal of Jiangsu University：Medicine Edition

基　　金：上海市科委发展基金重点资助项目(01JC14018);泰州市311工程人才基金资助项目(201036)

摘　　要：目的:研究IFN-γ对Thy1肾炎模型大鼠肾组织Sm ad7及Ⅳ型胶原表达的影响,从而为阐明IFN-γ抗肾纤维化机制及治疗肾纤维化提供理论和实验依据。方法:制备大鼠抗胸腺细胞血清(antithymocyte serum,ATS)肾炎模型,经尾静脉一次注射IFN-γ15万IU/kg,采用Northern b lot及免疫组化检测病变肾组织Sm ad7、Ⅳ型胶原的表达水平。结果:注射IFN-γ的ATS肾炎模型大鼠肾组织内Sm ad7的表达增加,Ⅳ型胶原mRNA的表达明显减少。结论:IFN-γ可能是通过上调Sm ad7表达而抑制肾组织Ⅳ型胶原表达的机制,对肾小球纤维化起抑制作用。Objective： To investigate the mechanism of IFN-γ antifibrosis and provide value clues for searching and developing new approaches to the prevention and treatment of organfibrosis,the expression of Smad7 and type Ⅳ collagen was detected in renal tissue of rat ATS glomerulonephritis.Methods： Rat antithymocyte serum glomerulonephritis model was built successfully adopting injection of IFN-γ 15×104 IU/kg vie tail vein once time.Northern blot and immunohistochemistry were employed to identify protein and mRNA expression of Smad7 and type Ⅳ collagen（Col Ⅳ） in renal tissue of rat ATS glomerulonephritis,respectively.Results： IFN-γ could increase the expression of Smad7 and decrease the expression of Col Ⅳ in renal tissue of rat ATS glomerulonephritis.Conclusion： It is possible that IFN-γ can alleviate the development of glomeruli fibrosis by the mechanism of upregulating the expression of Smad7,then downregulating the expression of Col Ⅳ.

关 键 词：系膜细胞 Γ干扰素 SMAD7 Ⅳ型胶原 信号转导 肾小球肾炎 

分 类 号：R365[医药卫生—病理学]