BDE-209和PCB153在致大鼠肝细胞DNA蛋白质交联(DPC)上的拮抗作用  

Antagonistic Effects on DPC Formation in Hepatocytes of Rats Induced by BDE-209 and PCB153

作　　者：李子文[1] 李师登[1] 张凤娇[1] 刘丹丹[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室,武汉430079

出　　处：《生态毒理学报》2011年第2期138-142,共5页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金杰出青年基金项目(50725620)

摘　　要：多溴联苯醚(PBDEs)和多氯联苯(PCBs)都是为人熟知的持久性有机污染物,也同为室内半挥发性有机物(SVOCs)。由于二者结构类似,通常认为PBDEs具有和PCBs相似的毒性。染毒剂BDE-209和PCB153分别是PBDEs和PCB家族的主要同系物,并且同时存在于环境和人体组织中。实验以DNA蛋白质交联(DPC)为生物标志物研究BDE-209和PCB153单独及联合染毒的遗传毒性。对大鼠肝细胞在37℃下染毒1h。浓度设置如下:BDE-209(0、1、6.5、12μg.mL-1);PCB153(0、1、6.5、12μg.mL-1);BDE-209(3.25μg.mL-1)+PCB153(3.25μg.mL-1)。实验结果显示:与溶剂对照相比,测得DPC水平随BDE-209(或PCB153)浓度增大而显著增高(p<0.01);PCB153比BDE-209引起更高水平的DPC(p<0.05或p<0.01);联合染毒组的DPC水平显著低于PCB153组(p<0.01),但稍高于BDE-209组(p>0.05)。从以上结果可知,BDE-209和PCB153之间存在拮抗作用;BDE-209和PCB153具有遗传毒性,并且就致DPC作用而言,二者之间可能存在拮抗作用。Polybrominated diphenyl ethers（PBDEs）and polychlorinated biphenyls（PCBs）are both well-known persistent compounds that belong to indoor semi-volatile organic compounds（SVOCs）.Usually,PBDEs are thought to have semblable toxicities to PCBs due to their structural similarity.In this research,BDE-209 and PCB153 were chosen as exposure substances that can coexist in the environment and human tissues as dominant congers of PBDEs and PCBs,respectively.To explore the genotoxicity of BDE-209 and PCB153 and interaction between them,DNA-protein crosslinks（DPC）were measured in hepatocytes treated with BDE-209（0,1,6.5,12 μg·mL-1）,PCB153（0,1,6.5,12 μg·mL-1）and BDE-209（3.25 μg·mL-1）＋ PCB153（3.25 μg·mL-1）at 37℃ for 1 h.Compared with control,the DPC levels induced by BDE-209 and PCB153 clearly increased along with their concentrations（p 0.01）.The results show that PCB153 induced DPC levels are higher than BDE-209（p 0.05 or p 0.01）.The DPC level induced by BDE-209＋PCB153 is significantly lower than PCB153（p 0.01）while a little higher than BDE-209（p 0.05）.Antagonistic effects between BDE-209 and PCB153 were observed.It can be concluded that BDE-209 and PCB153 can induce genotoxicity and the two chemicals have antagonistic effect each other for DPC formation in the hepatocytes of rats.

关 键 词：BDE-209 PCB153 DPC 大鼠肝细胞 拮抗作用 

分 类 号：X171.5[环境科学与工程—环境科学]