奶粉中V_(K_1)的超高效液相色谱定量和串联液质确证分析  

Determination of Vitamin K_1 in Infant Milk Powder by UPLC and LC-MS/MS

作　　者：冼燕萍 罗海英 罗东辉 郭茂章 陈意光 吴文海 

机构地区：[1]广州市质量监督检测研究院国家加工食品质量监督检验中心(广州),广东广州510110

出　　处：《食品研究与开发》2011年第4期130-133,共4页Food Research and Development

基　　金：广东省科技计划项目(2007B080401015);广东省科技计划项目(2007B023001001)

摘　　要：建立婴幼儿奶粉中VK1的超高效液相色谱(UPLC)检测方法和液相色谱-串联质谱(LC-MS/MS)检测确证方法。经研究,对国标检测方法的前处理过程稍作改进,减少基质干扰,操作简便,提取率稳定。UPLC法检出限为40μg/kg(S/N=3),LC-MS/MS法检出限为3.5μg/kg(S/N=3);样本添加水平在500μg/kg～2 000μg/kg时,平均回收率为78.4%～88.1%,相对偏差(n=6)为3.7%～6.4%。该方法能够满足奶粉中VK1含量的准确测定。Determination of Vitamin K1 in infant milk powder by UPLC and verified by HPLC-MS/MS were established.Compared to the current national standard method,the pre-treatment process was improved,and matrix interference was reduced.The method is simple and the extraction rate was stability.The detection limits of UPLC was 40 μg/kg（S/N=3）,The detection limits of HPLC-MS/MS was 3.5 μg/kg（S/N=3）;At the spiking levels of 500 μg/kg-2 000 μg/kg,the average recoveries were 78.4 %-88.1 % with RSD（n=6） values between 3.7 % and 6.4 %.The method can measure the levels of vitamin K1 in milk powder accurately.

关 键 词：超高效液相色谱 液相色谱-串联质谱 婴幼儿奶粉 VK1 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]