桃PGIP基因及其启动子的克隆与分析  

Isolation and Analysis of PGIP Gene and Its Promoter in Peach [Prunus persica (L.) Batch]

作　　者：王秀云[1] 张计育[1] 高志红[1] 章镇[1] 俞明亮[2] 张妤艳[2] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]江苏省农业科学院园艺研究所,南京210014

出　　处：《基因组学与应用生物学》2011年第2期159-167,共9页Genomics and Applied Biology

基　　金：国家自然科学基金(30871681);江苏省科技基础设施建设计划(BM2008008)共同资助

摘　　要：桃流胶病是一种严重危害桃树的真菌性病害,为研究PGIP基因在桃抗流胶病及抗其它真菌性病害中的作用,本研究以桃抗流胶病品种‘南京白沙’叶片为材料,对其PGIP基因及启动子序列进行克隆与分析。克隆测序获得了‘南京白沙’桃PGIP基因cDNA序列(GenBank登录号:HQ453972)和PGIP基因组DNA序列以及起始密码子上游453bp的启动子序列(GenBank登录号:HQ453974),并将该PGIP基因命名为PpPGIP2。‘南京白沙’桃PpPGIP2序列分析显示,该DNA序列具有完整的阅读框,无内含子,与GenBank中登录的李属PGIP基因序列同源性在93%～98%之间,与测序完成的桃全基因组中该基因的序列同源性为96%;PpPGIP2编码的氨基酸序列分析显示,该氨基酸序列含有2个典型的亮氨酸重复序列,信号肽为第1～第24个氨基酸残基;PGIP基因的序列聚类图显示,除了科、属、种间的同源性差异外,桃的种内PGIP基因同源性也有较大差异;PpPGIP2启动子序列分析发现3个抗病相关元件,分别为:GT1CONSENSUS、SEBFCONSSTPR10A和WBOXATNPR1,另外还有与激素调控、胁迫有关的调控元件。本研究对‘南京白沙’桃PGIP基因和启动子的克隆与分析,将为进一步研究桃PGIP基因的表达调控及其功能分析提供参考。Gummosis is one of the major fungi diseases occurred on peach tree.In order to study the role of PGIP gene playing on peach resistance to gummosis and other fungi diseases,we isolated and analysed the PGIP gene and its promoter from leaves of ＇Nanjing Baisha＇,a peach cultivar resisting gummosis.The cloning and sequencing results showed that the cDNA sequence （GenBank accession number：HQ453972） and the DNA sequence with an up-stream fragment of 453 bp length （GenBank accession number：HQ453974） from PGIP gene initiator codon of Prunus persica PGIP gene was amplified.The PGIP gene was named PpPGIP2.Sequence analysis showed that the cDNA sequence had complete open reading frame without intron,and was 93%～98% identical with the sequences of PGIP genes in Prunus and 96% identical with the genomic DNA of P.persica.The encoded amino acid sequence was a typical lecucine-repeated sequence and the predicted signal peptide was formed by 24 residues from 1 to 24.Besides family,genus and species,the clustering figure of PGIP genes showd that some diversities exsit in PGIP gene homology between peach species.Three disease resistant elements GT1CONSENSUS,SEBFCONSSTPR10A and WBOXATNPR1 were identified from PGIP promoter,which also has some hormone regulating and stress responsive elements.This study would contribute to further research on the regulation of expression and functional analysis of P.persica PGIP gene.

关 键 词：桃 PGIP基因 CDNA序列 启动子 调控元件 

分 类 号：S662.1[农业科学—果树学] Q943.2[农业科学—园艺学]