赤霉素对离体条件下玉米籽粒发育的影响  

Gibberellin's Influence on Corn's Kernal Development in Vitro

作　　者：刘立岩[1] 孙国伟[2] 

机构地区：[1]铁岭师范高等专科学校,辽宁铁岭112000 [2]铁岭农科院,辽宁铁岭112000

出　　处：《中国农学通报》2011年第9期116-119,共4页Chinese Agricultural Science Bulletin

摘　　要：为了探讨不同浓度的赤霉素GA对籽粒的生长发育的影响,笔者以玉米杂交种‘农单5号’和‘农大108’为材料,采用玉米籽粒离体培养的方法,对籽粒的发芽率、败育率、α-淀粉酶活性、干重和鲜重几个方面进行研究,结果表明:GA在低浓度(0～1mg/L)时,籽粒的发芽率、α-淀粉酶活性和败育率随着GA浓度的提高呈上升趋势,干重和鲜重都呈下降趋势;在较高浓度(1～20mg/L)时与上述情况正呈相反态势。当GA浓度为1mg/L时,籽粒的发芽率、α-淀粉酶活性和败育率分别达最大值;当GA浓度为20mg/L时,籽粒的发芽率、α-淀粉酶活性和败育率分别达最小值,而籽粒的鲜重、干重分别达最大值,并高于对照组。因此,授粉初期喷洒一定浓度(20mg/L)的GA对玉米产量有一定促进作用。To investigate the influence of gibberellin GA of different concentrations on kernal development,this experiment adopted the method of culture in vitro with the materials of maize hybrids of ＇ Nongdan NO.5 ＇ and ＇ Nongda 108 ＇,and focused on the gibberellin GA ＇ s influence on kernal ＇ s germination rate,abortion rate,α-amylase activity,dry weight and fresh weight.The results showed：in a low concentration of GA（0-1 mg/L）,kernal ＇ s germination rate,α-amylase activity and abortion rate raised with the increase of GA ＇ s concentration while the dry weight and fresh weight decreased;in a high concentration of GA（1-20 mg/L）,the situation reversed.While GA ＇ s concentration was 1 mg/L,kernal ＇ s germination rate α-amylase activity and abortion rate reached the maximum value.While GA ＇ s concentration was 20 mg/L,they dropped to the minimum value,but the dry weight and fresh weight reached to the maximum value in this concentration and higher than the control group.Thus the spraying of GA（20 mg/L） in pollination period will help the corn production in a certain degree.

关 键 词：赤霉素 离体培养 发芽率 败育率 Α-淀粉酶活性 干重 鲜重 

分 类 号：S311[农业科学—作物栽培与耕作技术]