正交试验法优化细叶黄皮叶中叶绿素的提取工艺  

Optimization of Chlorophyll Extraction Process from the Leaves of Clausena anisum-olens by Orthogonal Design

作　　者：苏秀芳[1] 卢祖辉[1] 陈昌强[1] 

机构地区：[1]广西民族师范学院化学与生物工程系,广西崇左532200

出　　处：《安徽农业科学》2011年第10期5730-5731,共2页Journal of Anhui Agricultural Sciences

基　　金：广西自然科学基金项目(2010GXNSFA013050)

摘　　要：[目的]优化细叶黄皮叶(Clausena anisum-olens)中叶绿素的提取方法,为细叶黄皮叶中叶绿素的分离纯化奠定基础。[方法]在单因素试验的基础上,采用L9(34)正交设计法优化细叶黄皮叶中叶绿素的提取工艺。[结果]微波萃取细叶黄皮叶中叶绿素的最佳工艺条件为:提取剂95%乙醇,料液比1∶10,温度70℃,功率为350 W,提取时间7 min。[结论]该试验优化的提取工艺简便、高效,为细叶黄皮叶中叶绿素的开发利用奠定了基础。[Object] To optimize the extraction process of chlorophyll from the leaves of Clausena anisum-olens,and provide basis for the purification of it.[Method] On the basis of single factor test,the extraction process of chlorophyll in the leaves of Clausena anisum-olens was optimized through orthogonal test.[Result]The optimum extraction process was as follows：extraction solvent of 95% alcohol,solid-liquid ratio of 1∶10,extraction temperature of 70 ℃,microwave power of 350 W,extraction time of 7 min.[Conclusion] The extraction process obtained in this study was simple and efficient,which lays foundation for the further development of chlorophyll in the leaves of Clausena anisum-olens.

关 键 词：细叶黄皮 叶绿素 正交试验 微波萃取法 

分 类 号：S567.23[农业科学—中草药栽培]