马铃薯花粉原生质体分离的研究  

Pollen Protoplasts Isolation in Solanum tuberosum

作　　者：王蒂[1] 司怀军[1] 王清[1] 

机构地区：[1]甘肃农业大学农学系,兰州730070

出　　处：《园艺学报》1999年第5期323-326,共4页Acta Horticulturae Sinica

基　　金：国家教委优秀年轻教师基金

摘　　要：以马铃薯减数分裂二分体和四分体时期的小孢子为材料， 对原生质体分离的主要影响因素进行了研究。结果表明， １ ％ 蜗牛酶和０ ．５ ％ 崩溃酶的混合酶液游离效果最好， 原生质体的最高得率达６５．４ ％ 。渗透压调解剂以蔗糖最好， 甘露醇次之， 最适浓度分别为１６％和１８％ 。０ ．１ ％ 荧光增白剂检测表明脱壁完全， ＦＤＡ 荧光检测表明花粉原生质体具有生活力。The pollen protoplast isolation from the microspores of Solanum tuberosum at dyad and tetrad stage of meiosis were studied.The result showed that 1% snailase and 0.5% driselase was the best enzyme combination for protoplast isolation,with a yield up to 65.4%.The best osmoticum is sucrose and the second is glucose and their optimum concentration was 16% and 18% respectively.Wall degraded pollens was identified with 0.1% calcoflower white test and its vitality was tested by fluorochromatic reaction with fluorescein diacetate(FDA).

关 键 词：马铃薯 花粉原生质体 分离 

分 类 号：S532[农业科学—作物学]