BVDV基因E_0在人参发根的转化及其分子检测  

Transformation and Molecular Detection of BVDV Gene E_0 in Ginseng Hairy Roots

作　　者：李然[1] 臧埔 郜玉钢[1] 马琳[1] 王亚星[1] 李学[1] 李萍[1] 张连学[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《中国农学通报》2011年第10期239-242,共4页Chinese Agricultural Science Bulletin

基　　金："梅花鹿高效养殖加工关键技术的研究与应用"(2009GJB10031);国家科技支撑计划项目"平地载参关键技术研究"(2007BAI38B01);中国博士后科学基金项目"鹿源牛病毒性腹泻病毒E0基因在人参发根中表达及免疫原性研究"(20090461042)

摘　　要：将重组鹿源BVDV基因E0植物表达载体(pBI121/E0),通过发根农杆菌Ri介导,用叶盘法转化得到人参发根,分别用PCR、RT-PCR检测其转化情况。结果表明:获得了转鹿源BVDV基因E0人参发根,鹿源BVDV基因E0已整合到人参发根基因组中并得到了转录,为研制转基因人参发根疫苗提供了科学依据和材料。Ginseng hairy roots were induced by leaf disc method,and recombinant plant expression vector（pBI121/E0）of BVDV geneE0 of deer was transformed into ginseng hairy roots byAgobacterium rhizgenses with Ri-plasmid.E0 in ginseng hairy roots was detected by PCR,RT-PCR methods.The results indicated that E0 transgenic ginseng hairy roots were obtained.E0 was transferred into Ginseng hairy roots,and expressed in ginseng hairy roots.

关 键 词：BVDV E0基因 人参发根 RT-PCR 

分 类 号：Q789[生物学—分子生物学]