肝癌组织中多种自体吞噬的形态测量学分析  

Morphometric analysis of various types of autophagy in hepatocellular carcinoma tissues

作　　者：王昊[1] 周东勋[1] 周华邦[1] 汪慧[1] 涂芊茜[1] 胡和平[1] 

机构地区：[1]第二军医大学东方肝胆外科医院肝脏内科,上海200438

出　　处：《第二军医大学学报》2011年第3期238-242,共5页Academic Journal of Second Military Medical University

摘　　要：目的调查不同类型自体吞噬在肝癌及癌旁组织中的分布情况。方法通过电子显微镜观察15对肝癌组织标本的超微结构,并使用相关软件量化图像指标以分析不同类型自体吞噬分布与细胞超微结构特征之间的联系。结果数据提示肝癌细胞中主要分布巨型自噬,分布与肿瘤细胞的活动及代谢情况相关;而癌旁细胞主要分布微型自噬,且分布与细胞的炎症损伤程度相关。结论巨型自体吞噬和微型自体吞噬在肝细胞癌组织及癌旁组织中呈现不对称分布,该现象可能与肝癌的发生相关。Objective To investigate the distribution of various types of autophagy in hepatocellular carcinoma（HCC） tissues and the adjacent non-tumor tissues.Methods We observed the ultrastructure of 15 HCC tissues and the matched adjacent non-tumor tissues under electron microscope.Scion Image software was used to analyze the relationship between distribution of various types of autophagy and cell ultrastructure.Results The data showed that macroautophagy was the dominant one in HCC cells,and the distribution was associated with the activity and metabolism of HCC cells.We also found that the microautophgy was the dominant one in the cells of adjacent tissues,and the distribution was associated with the inflammation injury of cells.Conclusion Our study suggests that macroautophagy and microautophagy are asymmetrically distributed between HCC cells and hepatocytes of the adjacent tissues,which might be related to the development of HCC.

关 键 词：自噬 亚细胞部分 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]