EBV LMP1和CMVpp65在肾脏疾病肾组织中的表达  

EBV LMP1 and CMVpp65 in renal tissue of patients with renal diseases

作　　者：余晓霞[1] 刘华锋[1] 陈孝文[1] 陶静莉[1] 杨陈[1] 李尚妹[1] 

机构地区：[1]广东医学院附属医院肾病研究所,湛江524001

出　　处：《临床与实验病理学杂志》2011年第3期257-260,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：湛江市科技攻关计划项目(2009C3103001)

摘　　要：目的 研究非洲淋巴细胞瘤病毒LMP1（EBV LMP1）、巨细胞病毒pp65（CMVpp65）在肾脏疾病患者肾组织中的表达情况,探讨病毒感染与人类肾脏疾病发生、发展的关系.方法 临床资料齐全的肾穿刺活检标本435例,免疫组化法检测EBV LMP1和CMVpp65在肾组织中的表达情况.结果 （1）175例（40.2%）肾组织中EBV LMP1表达阳性,狼疮性肾炎（LN）组及IgA肾病（IgAN）组与非免疫性肾病组及原发性肾炎（PGN）组比较,差异有统计学意义;170例（39.1%）肾组织中CMVpp65表达阳性,LN组与非免疫性肾病组及PGN组比较,差异有统计学意义;（2）PGN组初发患者与复发患者肾组织EBV LMP1、CMVpp65阳性率比较差异有统计学意义,其他病种则无;（3）合并感染与无合并感染患者EBV LMP1、CMVpp65阳性率比较差异均无统计学意义;（4）肾组织EBV LMP1表达阳性的LN患者血清anti-Sm-Ab阳性率高于阴性组（P〈0.05,OR=6.00,95%CI：1.200～29.998）;而CMVpp65表达阳性者血清anti-Sm-Ab阳性率低于阴性组（P〈0.01,OR=0.156,95%CI：0.038～0.643）.结论 肾组织EBV及CMV感染可能参与LN及IgAN的发病,其中EBV感染可能以分子拟态方式诱发系统性红斑狼疮.关键词：爱泼斯坦-巴尔病毒;人类巨细胞病毒;肾脏疾病;肾组织分类号：R737.1 文献标识码：A文章编号：1001-7399（2011）03-0257-04EBV LMP1 and CMVpp65 in renal tissue of patients with renal diseasesYU Xiao-xia LIU Hua-feng CHEN Xiao-wen TAO Jing-li YANG Chen LIPurpose To investigate the role of Epstein-Bar virus（EBV） and human cytomegalovirus（HCMV） infection in human renal diseases. Methods 435 cases of renal biopsies with complete clinical data were recruited. The expressions of LMP1 and CMVpp65 were detected by immunohistoehemieal staining. Results （ 1 ） LMP1 was identified in 175 cases out of all 435 cases, while CMVpp65 was identified in 170 cases, and the expression positive rate of EBV LMP1 in LN group and IgAN group were significant higher than that in PGN group（ P 〈 0. 01 ）, no-immune renal disease group（ P 〈 0. 05 ）, the expression positive rate of CMVpp65 in LN group were significant higher than that in PGN group and no-immune renal disease group （ P 〈 0. 05 ）. （2） EBV LMP1 and CMVpp65 expression rate were statistical different between immunodepressauts using or no in PGN subset, but not in other subsets. （3） There were not statistically different of EBV LMP1 and CMVpp65 expression rate between infection patients or no infection patients. （4） The positive rate of anti-Sm-Ab in patients with LN was much higher in renal EBV infection group than in non-infection group （ P 〈 O. 05, OR = 6. 00, 95% CI：1. 200 - 29. 998）, while it was lower in CMV infection group（ P 〈 0. 01, OR = 0. 156, 95% CI ： 0. 038 - 0. 643 ）. Conclutions Renal EBV and CMV infection may participate in the pathogenesis of LN and IgAN. And renal EBV infection may induce LN via the mechanism of molecular mimicry.

关 键 词：爱泼斯坦-巴尔病毒 人类巨细胞病毒 肾脏疾病 肾组织 

分 类 号：R737.1[医药卫生—肿瘤]