Folin-Ciocalteu比色法测定桦褐孔菌多酚的条件优化  

Optimization on determination of polyphenols from Inonotus obliquus by Folin-Ciocalteu colorimetry

作　　者：张梅梅[1] 魏志文[1] 刘玉冰[1] 赵艳霞[1] 郑维发[1] 

机构地区：[1]徐州师范大学江苏省药用植物生物技术重点实验室,徐州221116

出　　处：《菌物学报》2011年第2期295-304,共10页Mycosystema

基　　金：国家自然科学基金(No.31070052);江苏省自然科学基金(No.BK2010027&BK2009084);徐州师范大学自然科学基金(No.10XLA21)

摘　　要：以没食子酸为标准品,通过正交试验和单因素实验研究了Folin-Ciocalteu比色法测定桦褐孔菌中多酚含量的适宜条件。结果表明,优化后的显色条件为Folin-Ciocalteu试剂用量0.3mL、10%Na2CO3溶液0.6mL,25℃时避光反应30min,于750nm处测定其吸光值。多酚质量浓度在0.0032-0.0256mg/mL范围内与吸光值有良好的线性关系。根据拟合的线性回归方程对桦褐孔菌多酚进行定量测定,野生桦褐孔菌和人工培养桦褐孔菌中多酚含量最高分别为(72.05±0.08)mg/g、(52.76±0.06)mg/g(n=6)。不同加标水平的加标回收率测定实验获得的平均回收率为98.95%,RSD为1.27%。该法测定桦褐孔菌多酚具有快速、准确,稳定性强,重现性好,精密度高的特点,可应用于实际样品的测定。A quantitative method for the determination of polyphenols in sclerotia or mycelia of Inonotus obliquus was studied by orthogonal and single factor experiment with Folin-Ciocalteu reagent and standard of Gallic acid.The results showed that the content of polyphenols in I.obliquus can be well calculated according to their colorimetric absorption at 750nm by applying Folin-Ciocalteu reagent 0.3mL and 10% Na2CO3 0.6mL at 25℃ for 30min.The linear range of calibration curve was 0.0032-0.0256mg/mL for the gallic acid（r2=0.9975）.Using the optimezed method,the content of polyphenols in sclerotia was determinated to be（72.05±0.08）mg/g and mycelia to be（52.76±0.06）mg/g（n=6）.The average recovery was 98.95%（RSD=1.27%）.Therefore,the optimized method can dectect the content of polyphenols in I.obliquus in a swift,accurate and practical way with high reproducibility.

关 键 词：桦褐孔菌 多酚 Folin-Ciocalteu比色法 含量测定 

分 类 号：S567.3[农业科学—中草药栽培]