两株古尼拟青霉菌株的镇痛差异蛋白质组学研究  

Differential expression of analgesic proteins in two Paecilomyces gunnii isolates

作　　者：胡拓[1,2] 朱国胜[2,3] 刘永翔[2,3] 刘作易[2,3] 

机构地区：[1]贵州大学生命科学学院,贵阳550025 [2]贵州省农业生物技术重点实验室,贵阳550006 [3]贵州省农业科学院,贵阳550006

出　　处：《菌物学报》2011年第2期312-318,共7页Mycosystema

基　　金：贵州省农业科学院课题"古尼虫草活性成份研究"(院ZX[2007]035号)

摘　　要：为了探明古尼虫草无性型——古尼拟青霉差异表达蛋白与镇痛活性之间的关系,对有镇痛活性和无镇痛活性的两个古尼拟青霉菌株,进行蛋白质差异表达研究,结果显示,活性菌株(HZJ-1)中出现或表达显著上调的蛋白质点数为51个,结构鉴定得到39个类别功能各异的蛋白质,其中大量蛋白与代谢相关,其中NADP特异性谷氨酸脱氢酶可能参与使长时程突触传递的效能减弱的作用,从而减弱了疼痛的发生。另外几个保守假定蛋白的功能尚未鉴定,还有待进一步研究。Two isolates of Paecilomyces gunnii,anamorph of Cordyceps gunnii,with and without producing analgesic activity,were tested by using proteomic analysis in order to find the correlation between protein differential expression and analgesic activity.The results showed that expression of 51 protein spots significantly increased in the active isolate HZJ-1.Among the spots,structures of 39 proteins with various functions were identified.Most of the proteins were related to metabolism,and the NADP-specific glutamate dehydrogenase might mediate long-term depression of synaptic transmission and reduce pain.The functions of several conserved hypothetical proteins have not been reported,and are needed for further studies.

关 键 词：古尼拟青霉 镇痛活性 蛋白表达差异 谷氨酸脱氢酶 

分 类 号：R379[医药卫生—病原生物学] Q93[医药卫生—基础医学]