犬源韦氏巴贝斯虫的分子鉴定和系统发育研究  

Molecular identification and phylogenetic analysis of Babesia vogeli from dogs

作　　者：李高强[1,2] 张浩吉[2] 张更利[2] 蒲文珺[2] 周庆国[2] 丁巧玲[2] 李金平[1,2] 李国清[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]佛山科学技术学院动物医学系,广东佛山528231

出　　处：《中国兽医科学》2011年第3期245-249,共5页Chinese Veterinary Science

基　　金：广东省自然科学基金项目(10152800001000007)

摘　　要：无菌采集疑似巴贝斯虫感染血样,进行全血基因组DNA的抽提,用保守引物5-22F和1661R进行巴贝斯虫18SrRNA基因的PCR扩增,扩增产物经克隆、测序后,获得大小为1 677bp的核苷酸序列。序列比对和系统发育分析发现,该序列与韦氏巴贝斯虫(AB083374)18SrRNA基因的相似性达100%,而与犬巴贝斯虫(AY072926)、罗氏巴贝斯虫(L19079)和吉氏巴贝斯虫(AF271081)18SrRNA基因的相似性分别为98.0%,95.2%和93.4%。从分子水平证实了韦氏巴贝斯虫在广东宠物犬的存在,同时也对巴贝斯虫的分类进行了讨论。The 18 S rRNA gene of Babesia was amplified by PCR using a pair of conserved primers（5-22F and 1661R） from canine whole blood genomic DNA extracted from suspected pet dogs in Guangzhou and Foshan.The recombinant plasmids were identified by PCR and the positive clones were sequenced.A 1 677 bp sequence in length was obtained that was identical to the 18 S rRNA gene of Babesia vogeli published previously（AB083374）,meanwhile,compared with the 18 S rRNA gene of B.canis（AY072926）,B.rossi（L19079） and B.gibsoni（AF271081） available in GenBank,the sequences similarities were 98.0%,95.2% and 93.4%,respectively.We confirmed the prevalence of B.vogeli in pet dogs in China by molecular approach for the first time,and the classification of Babesia was discussed in the present study.

关 键 词：犬 韦氏巴贝斯虫 18 S RRNA基因 系统进化分析 

分 类 号：S852.723[农业科学—基础兽医学]