P53和Ki67在胃癌中的表达及其临床意义  

Clinical significance of P53 and Ki67 expression in gastric cancer

作　　者：刘伟[1] 余英豪[1] 欧阳学农[2] 王烈[3] 武一曼[4] 陈娟[1] 熊喜生[1] 

机构地区：[1]中国人民解放军南京军区福州总医院病理科,福建省福州市350025 [2]中国人民解放军南京军区福州总医院肿瘤科,福建省福州市350025 [3]中国人民解放军南京军区福州总医院普外科,福建省福州市350025 [4]福建中医药大学病理教研室,福建省福州市350003

出　　处：《世界华人消化杂志》2011年第4期367-373,共7页World Chinese Journal of Digestology

摘　　要：目的:探讨胃癌中P53和Ki67蛋白表达与临床病理参数的相关性及其临床意义.方法:收集在中国人民解放军南京军区福州总医院手术切除的胃癌组织标本1107例,采用EliVisionTM plus免疫组织化学染色方法检测其中临床资料完整的775例胃腺癌标本中Ki67和P53蛋白表达.结果:在775例胃腺癌组织标本中,P53和Ki67蛋白的阳性表达率分别为46.84%和78.97%,在非肿瘤性胃黏膜中均未见其阳性表达;P53和Ki67蛋白表达,在肠型胃癌中显著高于弥漫型胃癌(54.7%vs38.0%;86.0%vs70.0%,均P<0.05),高中分化胃癌显著高于低分化胃癌(54.7%vs35.4%;87.0%vs67.2%,均P<0.05);P53蛋白表达在胃食管连接部胃癌和无远处转移的胃癌中显著高于胃部胃癌和有远处转移的胃癌(53.7%vs41.9%;49.0%vs30.3%,均P<0.05);Ki67蛋白表达随着浸润深度的增加而增高(80.3%vs60.4%,P<0.05),有淋巴结转移的患者显著高于无淋巴结转移的患者(81.4%vs72.6%,P<0.05).结论:联合检测P53和Ki67蛋白表达有助于阐释胃癌发生发展、浸润转移的机制,并可作为评估胃癌恶性生物学行为及预后的参考指标.AIM：To investigate the correlation of P53 and Ki67 protein expression with the clinicopathological parameters in gastric cancer.METHODS：A total of 1 107 surgical specimens of gastric cancer were collected from the Fuzhou General Hospital of Nanjing Military Command,The expression of P53 and Ki67 was examined by immunohistochemistry in 775 gastric cancer specimens taken from patients with complete clinical data.RESULTS：The positive rates of P53 and Ki67 protein expression were 46.84%and 78.97%,respectively.Higher positive rates of P53 and Ki67 protein expression were noted in intestinal-type gastric cancer than in diffuse-type gastric cancer（54.7%vs 38.0%,86.0%vs 70.0%,both P0.05） and in well-differentiated cancer than in poorly-differentiated cancer（54.7 vs 35.4%,87.0%vs 67.2%,both P0.05）.A higher positive rate of P53 protein expression was noted in gastroesoph-ageal junction（GEJ）cancer than in gastric cancer（53.7%vs 41.9%,P0.05）and in cancer without distant metastasis than in that with distant metas-tasis（49.0%vs 30.3%,P0.05）.A higher positive rate of Ki67 protein expression was noted in inva-sive cancer than in less invasive cancer（80.3%vs 60.4%,P0.05）and in cancer with lymph node metastasis than in that without lymph node metastasis（81.4%vs 72.6%,P0.05）.CONCLUSION：P53 and Ki67 protein expression might be associated with the development,invasion,and metastasis of gastric cancer,and can be used to evaluate the malignant behavior and prognosis of gastric cancer.

关 键 词：胃癌 P53 KI67 表皮生长因子受体-2 免疫组织化学 临床病理参数 

分 类 号：R735.2[医药卫生—肿瘤]