IL-10修饰的树突状细胞在大鼠角膜移植中的作用  

Effects of IL-10 modified dendritic cells in rat corneal transplantation

作　　者：苏海英[1] 刁玉梅[1] 李兵[1] 

机构地区：[1]辽宁医学院附属第一医院眼科,中国辽宁省锦州市121001

出　　处：《国际眼科杂志》2011年第4期588-590,共3页International Eye Science

基　　金：中国辽宁省教育厅重点实验室资助项目(No.IS2010103)~~

摘　　要：目的:探讨IL-10修饰的供体树突状细胞(dendritic cells,DC)在大鼠角膜移植中的作用。方法:建立Wistar-SD大鼠穿透性角膜移植动物模型,Wistar大鼠为供体,SD大鼠为受体。受体大鼠42只随机分为3组,每组14只。A组为对照组,受体不经任何预处理,即行角膜移植术;B组角膜移植前3d,每只受体鼠经尾静脉注射供体的未成熟DC,细胞数为2×107个;C组角膜移植前3d,每只受体鼠经尾静脉注射经IL-10修饰的供体未成熟DC,细胞数为2×107个。结果:以混浊、水肿和新生血管3项指标作为临床评估标准,观察各组角膜植片的存活时间。角膜植片的存活时间3组分别为:10.1±1.7,17.8±1.8,26.4±1.6d。各干预组与对照组比较差异均有显著性(P<0.01)。B,C组间比较差异有显著性(P<0.01)。结论:摄取供体抗原的不成熟DC能显著延长角膜植片的存活时间;IL-10修饰的DC可进一步延长角膜植片的存活时间。AIM：To study the effect of donor IL-10 modified dendritic cells（DC）for providing theoretical and practical basis for anti-rejection of corneal transplantation.METHODS：Wistar rats were used as donors,and SD rats were used as recipients.The recipient was injected by tail vein with donor IL-10-treated DC or with untreated DC.Keratoplasty was performed after three days and the survival time of corneal allografts was observed.Animals were randomly assigned to the following three groups：（A）control,（B）DC,（C）IL-10-DC.RESULTS：The mean survival time in the control group was merely 10.1±1.7 days,but in the pretreated groups was 17.8±1.8 days and 26.4±1.6 days respectively which was statistically prolonged compared with the control groups（P〈0.01）,and comparison within the two pretreated groups was significantly different（P〈0.01）.CONCLUSION：It is indicated that pretreatment with donor immature DC could markedly prolong the survival time of corneal allografts.And IL-10-modified DC can further extend the rat corneal allografts.

关 键 词：IL-10 树突状细胞 角膜移植 

分 类 号：R779.65[医药卫生—眼科]