Fas相关死亡域样白介素-1β转换酶抑制蛋白在结直肠癌中的表达意义  

Expression and clinical significance of FLIP(S) mRNA in colorectal carcinoma

作　　者：梁贤明[1] 张忠英[1] 黄如欣[1] 

机构地区：[1]厦门大学附属中山医院临床检验中心,福建厦门361004

出　　处：《检验医学与临床》2011年第8期932-934,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨结直肠癌Fas相关死亡域样白介素-1β转换酶抑制蛋白(FLIP)(S)mRNA的表达水平及其临床意义。方法建立检测FLIP(S)mRNA荧光定量逆转录聚合酶链反应(FQ-RT-PCR),用该方法检测结直肠癌组织及其癌旁正常组织FLIP(S)mRNA表达水平。结果 FLIP(S)mRNA在结直肠癌组织和癌旁正常组织都有表达,在结直肠癌组织表达水平为0.23±0.19,明显高于癌旁正常组织0.13±0.10(P<0.05)。FLIP(S)mRNA表达水平与结直肠癌组织学分级密切相关(P<0.05),肿瘤分化程度越低,FLIP(S)mRNA的表达水平越高。结论 FLIP(S)在结直肠癌组织中表达升高;在分化越低组织中其表达水平越高;为揭示结直肠肿瘤的发生、发展提供新的理论依据,并为结直肠癌的有效治疗提供新的思路。Objective To investigate the expression and clinical significance of FLIP（S）mRNA in colorectal carcinoma.Methods A real-time fluorescence quantitative RT-PCR for FLIP（S）mRNA was established.Then the expression of FLIP（S）mRNA in colorectal carcinoma tissues and adjacent tissues was tested by the method.Results FLIP（S）mRNA was detectable in both tumor tissue and adjacent tissue.The expression level of FLIP（S）in colorectal carcinoma tissue（0.23±0.19）was significantly higher than that in adjacent colorectal tissue（0.13±0.10）（P0.05）.The different expression of FLIP（S）mRNA in colorectal carcinoma was significantly associated with histological grade（P0.05）.The poorer tumor differentiation became,the greater expression level of FLIP（S）mRNA was.Conclusion The expression of FLIP（S）mRNA is up-regulation and closely related to the malignant in colorectal carcinoma tissue,It may help to explore the genesis and development of colorectal carcinoma and find an effective therapeutic method for the tumor.

关 键 词：结直肠癌 聚合酶链反应 Fas相关死亡域样白介素-1β转换酶抑制蛋白 

分 类 号：R743.3[医药卫生—神经病学与精神病学]