形态硒的研究过程中硒代蛋氨酸的稳定性研究  

Stability of Selenomethionine during the Extraction of Selenium Species

作　　者：赵秋香[1] 冯超[1] 陈福强[1] 

机构地区：[1]广东省物料实验检测中心,广东广州510080

出　　处：《广州化工》2011年第8期66-68,119,共4页GuangZhou Chemical Industry

摘　　要：运用高效液相色谱(HPLC)-小体积微波消解(LVMMD)-氢化物发生原子荧光光谱(HG-AFS)对不同情况下硒蛋氨酸(SeMet)的稳定性进行了分析,重点研究了传统的水浴振荡提取和方便快速的超声提取过程中SeMet的稳定性。实验表明:在水溶性SeMet的提取过程中,SeMet在37℃水浴振荡提取过程中很稳定;当提取时间小于30 min时,SeMet分子在超声提取过程中是相对稳定的。在酶溶性SeMet的提取过程中,水浴振荡和超声提取过程会引起酶的多肽链断裂或空间构型的改变,但是两个提取过程对PronaseE的影响不一样。而SeMet容易和这些变件后的酶或其中一部分结合形成新的存在形式,从而导致了不同提取过程后,SeMet洗脱时间发生不同的改变。Stabilities of selenomethionine （SeMet） were analyzed in different situations by High Performance Liquid Chromatography （HPLC） -Low Volume Microwave Digestion （LVMWD） -Hydride Generation -Atomic FlorescenceSpectrometry （HG - AFS）. Effects of the most widely used extraction procedures of Se species （water bath shaking anduhrasonic extraction） to the stabilities of SeMet were studied emphatically. The results showed that SeMet was stable whenwater bath （37 ℃ ） shaking was used for water leaching of Se species, and when the extraction time was less than 30 rain,SeMet was relatively stable by ultrasonic extraction too. During the enzymatic hydrolysis, Polypeptide chain rupture orspace configuration changes of Pronase E caused by by external forces （water bath shaking or sonication）. The effects ofwater bath shaking and ultrasonic extraction to the enzyme were different, and SeMet was easily combined with the de-formed enzyme or parts of it, which caused the different transfers of retention time of SeMet.

关 键 词：硒蛋氨酸 水提取 酶解 稳定性 

分 类 号：O657.31[理学—分析化学]