石油对翅碱蓬生长及生理特性的影响  

The Influence of Oil Concentration in Soil on Growth and Physiological Characteristics of Suaeda heteroptera

作　　者：何洁[1,2] 贺鑫[1,2] 高钰婷[1,2] 王斌[3] 周一兵[3] 

机构地区：[1]大连海洋大学海洋环境工程学院,辽宁大连116023 [2]大连海洋大学近岸海洋环境科学与技术辽宁省高校重点实验室,辽宁大连116023 [3]大连海洋大学生命与科学技术学院,辽宁大连116023

出　　处：《农业环境科学学报》2011年第4期650-655,共6页Journal of Agro-Environment Science

基　　金：国家"863"计划项目(2006AA10Z410);国家海洋局公益项目(200805069);辽宁省教育厅项目(2009A167)

摘　　要：通过盆栽试验,测定不同石油浓度下翅碱蓬的生长生理指标及土壤中石油含量的变化,研究石油对翅碱蓬萌发、生长、生理特性和抗氧化酶系统的影响及翅碱蓬对土壤中石油的降解。结果表明,随着石油含量的升高,翅碱蓬种子发芽率、苗高、苗重和苗中叶绿素、粗蛋白含量逐渐下降,电导率上升,石油浓度在5000mg.kg-1以上,影响显著。石油对翅碱蓬糖的合成有明显抑制作用。当石油浓度为1000~5000mg.kg-1时,翅碱蓬抗氧化酶能够快速、长效地提高活性来抵御逆境,使其不受到伤害或减轻伤害,可溶性蛋白保持在高水平;当石油浓度达到10000mg.kg-1时,抗氧化酶系统迅速作出反应,酶活性急剧提高,但因毒性超出了其可以承受的范围,而无法维持较长时间,随后会降至对照组以下,同时可溶性蛋白也急剧下降,仅为对照组的9。石油浓度为1000~5000mg.kg-1时,石油去除率在63.53~83.21,达到10000mg.kg-1时,则下降到40.14。The aim of this paper was to determine some growth and physiological indexes of Suaeda heteroptera at different oil concentration and variation of oil concentration of soil,and study the effects of oil on germination,growth,physiological characteristic and the antioxidant system of Suaeda heteroptera and the removal of oil in soil by the experiment.The results indicated that with the concentration of oil increasing,the germination rates of the seed,the heights and weights of the plant,the contents of chlorophyll and crude protein in the plants decreased gradually,and the data of electrical conductivity increased,the harms of Suaeda heteroptera were significant in the concentration of oil over 5 000 mg·kg-1 or more.The synthesis of soluble sugar of Suaeda heteroptera was remarkable restrained by oil.The antioxidant enzymes could improve activity rapidly and long-term to against adversity rapid in the concentration of oil 1 000～5 000 mg·kg-1,it could make Suaeda heteroptera to avoid or reduce the damages,and make the contents of soluble protein keep a high level.The antioxidant enzymes activity was increased sharply,but no lasting longer owing to oil toxicity beyond the scope of Suaeda heteroptera＇s affordable in the concentration of oil 10 000 mg·kg-1,the enzymes activity would decrease below the control groups soon,meanwhile the contents of soluble protein sharply dropped to only 9% of the control groups.The removal rates of oil were 63.53%～83.21% in the concentration of oil 1 000～5 000 mg·kg-1,but it was only 40.14% in 10 000 mg·kg-1.

关 键 词：翅碱蓬 石油污染 生理指标 抗氧化酶系统 

分 类 号：X173[环境科学与工程—环境科学]