磷酸氯喹对白血病细胞株的影响  

The effect of chloroquine phosphate on leukemia cell lines

作　　者：刘佳[1] 陈芳源[1] 王海嵘[1] 钟华[1] 钟济华[1] 王利民[2] 欧阳仁荣[1] 

机构地区：[1]上海交通大学医学院附属仁济医院血液科,上海200001 [2]上海交通大学医学院附属仁济医院中心实验室,上海200001

出　　处：《中国实验诊断学》2011年第4期571-575,共5页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目(30670881)

摘　　要：目的本实验以药物磷酸氯喹干预白血病细胞株,观察其对白血病细胞生长凋亡的影响,同时研究用药后凋亡相关蛋白Pnas-2的变化,以探寻磷酸氯喹作用白血病细胞株机制。方法 MTT法检测药物干预后白血病细胞增长情况;AnnexinⅤ/sytox标记细胞以流式细胞仪检测药物干预前后细胞凋亡情况的差别;激光共聚焦观察蛋白Pnas-2的亚细胞定位,蛋白印迹实验检测药物作用前后,Pnas-2表达量的变化。结果 50μg/mL的磷酸氯喹能够诱导白血病细胞株凋亡。并发现凋亡相关蛋白Pnas-2在白血病细胞株中存在异常定位和过量表达。磷酸氯喹能够使白血病细胞株中Pnas-2蛋白的表达量及亚细胞定位发生变化。结论磷酸氯喹能够抑制白血病细胞株生长,诱导凋亡,机制可能是恢复了Pnas-2蛋白的异常定位及表达。Objective In this study,we treated leukemia cell lines with chloroquine phosphate to observe its effect on the cell lines.Besides we explored changes of the protein Pnas-2 after the intervention of the drug,in order to found the mechanism of the drug that influenced leukemia cell lines.Methods Chloroquine phosphate of defferent concentrations was added into the culture fluid at logarithmic phase.MTT assay was used to measure the cell proliferation,flow cytometry was applied to detect the cell apoptosis,immunofluorescence technology was employed to observe the subcellular location of protein Pnas-2,and Western blot was used to compare the expression content of Pnas-2.Results 50 μg/mL chloroquine phosphate could induce leukemic cell lines to apoptosis.And in leukemic cell lines,protein Pnas-2＇s expression content was more than non-tumor cell lines,and its subcellular location was abnormal,too.Simultaneously,the drug could revert Pnas-2＇s abnormal subcellular location and its redundant expression content.Conclusion chloroquine phosphate could inhibit the growth of leukemic cell lines and induce apoptosis.The mechanism may be related to its reversion of Pnas-2＇s abnormal content and location.

关 键 词：磷酸氯喹 白血病细胞株 PNAS-2 凋亡 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]