白细胞介素-6基因启动子-572C/G、-634C/G的多态性与脑梗死的研究  

Association of interleukin-6 gene promoter-572C/G and-634C/G polymorphisms in patients with cerebral infarction

作　　者：潘云[1] 韦叶生[1] 黄建敏[2] 覃羽华[1] 潘国刚[1] 

机构地区：[1]右江民族医学院附属医院检验科,广西百色533000 [2]右江民族医学院神经内科,广西百色533000

出　　处：《中国实验诊断学》2011年第4期627-630,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然基金资助项目(No:30660159)

摘　　要：目的探讨脑梗死患者白细胞介素-6（IL-6）基因启动子-572C/G-、634C/G的多态性及其血清水平与脑梗死的关系。方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的分析方法,检测了106例脑梗死患者和92例健康对照者IL-6基因多态性,同时采用酶联免疫吸附试验检测IL-6的血清水平。结果脑梗死组IL-6水平显著高于对照组（P〈0.05）,IL-6-572C/G基因型和等位基因频率在脑梗死组和对照组比较,差异有显著性（P〈0.05）,与健康对照组相比,发现G等位基因携带者患脑梗死的相对风险度增加1.634倍（OR=1.634,95%CI=1.135~2.468）。携带G等位基因的脑梗死患者血清IL-6水平显著高于不携带者（P〈0.05）。IL-6基因-634C/G多态性在脑梗死组和对照组之间比较差异无显著性（P〉0.05）。结论 IL-6基因-572C/G多态性与脑梗死之间存在相关关系,其中G等位基因可能是脑梗死发病的遗传易感基因;携带G等位基因的个体可能通过促进IL-6的高度表达进而增加了脑梗死的发病风险。Objective To study polymorphism of the interleukin-6（IL-6） gene promoter-572C/G and-634C/G in patients with cerebral infarction,and to study the relation between serum levels and genotype of interleukin-6.Methods The polymorphism of IL-6 was detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method in 106 case cerebral infarction patients and 92 healthy persons,The serum level of IL-6 was determined by enzyme-linked immunosorbent assay（ELISA）.Results cerebral infarction group showed significantly higher serum level of IL-6 than control group（P0.05）.There was a significant difference in frequencies of allele and genotype in IL-6 gene-572C/ G polymorphism between cerebral infarction and control groups（P0.05）.IL-6 allele G carriers increased onset risk with 1.634 fold for cerebral infarction（OR=1.634,95%CI=1.135～2.468） compared with healthy persons.The distribution of IL-6 gene-634C/G polymorphism was not different between cerebral infarction group and control group（P0.05）.IL-6 gene-572C/G polymorphism is associated with cerebral infarction The IL-6 allele G carriers may be at increased risk of cerebral infarction due to enhanced the IL-6 expression.

关 键 词：脑梗死 白细胞介素-6 基因多态性 

分 类 号：R743.33[医药卫生—神经病学与精神病学]