应用尿液蛋白质组技术筛选多发性骨髓瘤相关蛋白的初步研究  

Preliminary analysis of screening multiple myeloma-associated proteins by urinary proteomics

作　　者：房亚哲[1] 刘津[2] 吴琳[3] 娄晓敏[3] 赵冠飞[1] 张洪涛[1] 陈文明[4] 王清涛[1] 

机构地区：[1]首都医科大学附属北京朝阳医院检验科,北京100020 [2]海洋石油总医院 [3]中国科学院北京基因组研究所,北京100029 [4]首都医科大学附属北京朝阳医院血液科,北京100020

出　　处：《中国实验诊断学》2011年第4期647-650,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目(课题编号30872391)

摘　　要：目的应用蛋白质组学相关技术筛选多发性骨髓瘤(MM)患者尿液中差异表达的蛋白质,寻找与MM诊断相关的疾病标志物。方法收集MM组、疾病对照组(肾脏疾病)、健康对照组尿液各8例,TCA沉淀法提取尿液蛋白质后建立双向凝胶电泳图谱;Image Master软件筛选差异蛋白质;利用MALDI-TOF-MS获得肽质量指纹图谱,通过Mas-cot软件进行数据库搜索,得到蛋白质鉴定结果。结果筛选出57个差异蛋白点,43个在MM组上调,14个下调。对其中30个差异蛋白点进行分析,鉴定出甘露聚糖结合凝集素相关蛋白19(Map19)、人组织相容性抗原A2(Hla-A2)、视黄醇结合蛋白(RBP)、转甲状腺素蛋白(TTR)、免疫球蛋白轻链、α2-糖蛋白1(ZAG1)等9类差异表达的蛋白质。结论应用尿液蛋白质组技术成功筛选鉴定出MM尿液中存在的差异蛋白质,可能是MM疾病诊断相关标志物,尚需要进一步验证。Objective To screen and identify multiple myeloma-associated proteins in urine.Methods Collect the second morning urine of volunteers in MM group,disease control group（nephropathy） and healthy control group.Proteins were precipitated with TCA,and separated with two-dimensional polyacrylamide gel electrophoresis（2D-PAGE）.After silver nitrate staining,proteins differential expression was analyzed by Image Master software and identified with MALDI-TOF-MS.Results 57 proteins that were differentially expressed in MM comparedwith control groups and 9 proteins were identified including Mbl-associated protein 19（Map19）,Hla-A2,Retinol-binding protein（RBP）,transthyretin（TTR）,immunoglobulin light chain,alpha-2-glycoprotein 1（ZAG1） and so on.Conclusion Urinary proteomics is a successful tool to study the differentially expressed proteins,some of them might be the biomarkers of MM.

关 键 词：多发性骨髓瘤 尿液 蛋白质组学 MALDI-TOF-MS 

分 类 号：R733.3[医药卫生—肿瘤]