长春地区无偿献血者HPA1-6,15基因分型及频率调查  

Genetic frequency distribution of HPA1-6,15 in ChangChun in China

作　　者：于江虹[1] 杨帆[1] 焦立新[1] 刘铁梅[2] 

机构地区：[1]长春市中心血站输血研究所,吉林长春100033 [2]吉林大学中日联谊医院输血科,吉林长春130033

出　　处：《中国实验诊断学》2011年第4期680-682,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省卫生厅资助;项目编号(2009Z029);吉林省科技厅资助;项目编号:201015186

摘　　要：目的调查北方汉族人群人类血小板抗原(HPA)的基因频率及分布规律。建立一支HPA-1a,-2a,-4a,-5a,-6a阴性及已知HPA抗原的献血者队伍。方法随机抽取172名长春地区固定无偿血小板捐献者静脉血3ml,枸橼酸钠抗凝;采用TIANGEN试剂盒提取DNA。基因定量仪测定DNA的浓度和纯度。采用PCR-SSP方法进行试验。最后进行统计学分析。结果各表现型的频率分别是:HPA-1aa 0.988,HPA-1ab 0.012,HPA-2aa 0.833,HPA-2ab 0.159,HPA-2bb 0.008,HPA-3aa 0.355,HPA-3ab 0.482,HPA-3bb 0.163,HPA-4aa 0.988,HPA-4ab 0.012,HPA-5aa 0.971,HPA-5ab0.029,HPA-6aa 0.977,HPA-6ab 0.023,HPA-15aa 0.286,HPA-15ab 0.498,HPA-15bb 0.216。未发现HPA 1bb、4bb、5bb、6bb。各基因型的频率分别是:HPA-1a 0.994,HPA-2a 0.913,HPA-3a 0.596,HPA-4a 0.994,HPA-5a 0.985,HPA-6a 0.988,HPA-15a 0.535。其中基因频率占99%以上的有HPA-1a-、4a。各基因型的不配合率分别是HPA-1a 0.011,HPA-2a0.147,HPA-3a 0.366,HPA-4a 0.011,HPA-5a 0.028,HPA-6a 0.023,HPA-15a 0.374。结论本研究有助于在长春地区建立已知HPA抗原的机采血小板捐献者队伍,可以提高血小板抗体的检出率,为同种免疫性血小板减少症患者提供HPA相合的血小板。Objective This study is devised to discover the frequencies and principle of HPA genes in Han ethinity in North East of China and to build a donor group with HPA-1a,-2a,-4a,-5a,-6a negative or known HPA antigen types.Methods We randomly collected 172 blood samples from regular paletlet donors,distracted DNA using TIANGEN kit,measured DNA concentration and purity,tested using PCR-SSP then did the statistic analysis.Results The frequency of each HPA phenotype is HPA-1aa 0.988,HPA-1ab 0.012,HPA-2aa 0.833,HPA-2ab 0.159,HPA-2bb 0.008,HPA-3aa 0.355,HPA-3ab 0.482,HPA-3bb 0.163,HPA-4aa 0.988,HPA-4ab 0.012,HPA-5aa 0.971,HPA5ab 0.029,HPA-6aa 0.977,HPA-6ab 0.023,HPA-15aa 0.286,HPA-15ab 0.498,HPA-15bb 0.216,respectively.We didn,t find HPA-1bb、-4bb、-5bb、-6bb.The frequency of each HPA genotype is HPA-1a 0.994,HPA-2a 0.913,HPA-3a 0.596,HPA-4a 0.994,HPA-5a 0.985,HPA-6a 0.988,HPA-15a 0.535,respectively.Among them,the frequency of HPA-1a、-4a is above 99%.The mismatch rate of each genotype is HPA-1a 0.011,HPA-2a 0.147,HPA-3a 0.366,HPA-4a 0.011,HPA-5a 0.028,HPA-6a 0.023,HPA-15a 0.374,respectively.Conclusion This study helps to build a donor group with known HPA antigen types.It will help to promote the detection rate of platelet antibody and to supply the patients with HPA-matched platelets.

关 键 词：人类血小板抗原 基因型 表现型 

分 类 号：R440[医药卫生—诊断学]