哺乳动物雷帕霉素靶蛋白和存活蛋白在人脑胶质瘤中表达的研究  

Clinical study on expression of mTOR and survivin in human gliomas

作　　者：李鑫[1] 贾爱华[2] 任玉波[2] 许士明[3] 田秀丽[1] 赵存友 李学元[1] 

机构地区：[1]聊城市人民医院神经外科,山东省252000 [2]聊城市人民医院病理科,山东省252000 [3]聊城市人民医院放射科,山东省252000 [4]广州市香港科大霍英东研究院应用基因组中心

出　　处：《中华临床医师杂志（电子版）》2011年第5期65-68,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：广东省自然科学基金资助项目(10179942783X000002)

摘　　要：目的研究哺乳动物雷帕霉素靶蛋白(mTOR)和存活蛋白(survivin)在胶质瘤中的表达及二者的相关性。方法应用半定量免疫组织化学法分别检侧87例胶质瘤(Ⅰ～Ⅱ级27例,Ⅲ级24例,Ⅳ级36例)组织中pmTOR和survivin的表达,分析二者与肿瘤恶性程度的关系以及它们之间的相关性。结果 pmTOR和survivin在不同级别的胶质瘤中均有表达,阳性表达率差异无统计学意义(P>0.05),但其表达强度随着肿瘤病理级别的增高而升高,差异有统计学意义(P=0.0000),且pmTOR和survivin的表达强度呈正相关(r=0.858,P<0.00001)。结论 mTOR和survivin在胶质瘤的发生、发展中起着重要的作用,有望成为基因治疗的有效靶点。Objective To investigate expression of mTOR and survivin in 87 Chinese patients with gliomas and correlation of the two proteins was studied.Methods Semi-quantitative immunohistochemistry was used to examine the expression and activation level of pmTOR and survivin in tissues from 87 chinese patients with gliomas（grade Ⅰ-Ⅱ 27,grade Ⅲ 24 and grade Ⅳ 36）.The expression rates and activation level of the two proteins markers were associated with neoplasms′ malignancy grade,and the associaton of the two proteins was analyzed too.Results The activation level of of pmTOR and survivin showed significantly variance in higher grade astrocytomas than in lower ones（P=0.0000）,while the expression rate of them had no obviously differce in gliomas of grade Ⅰ-Ⅱ,grade Ⅲ and grade Ⅳ （P〉0.05）.There was a positive correlation between the expressive intensity of pmTOR and survivin （P〈0.01）.Conclusions mTOR and survivin play a cooperative role in the process of human gliomas and may serve as useful markers for predicting neoplasms′ biological behavior,furthermore they and can be useful target for gene therapy.

关 键 词：神经胶质瘤 他克莫司结合蛋白质类 存活蛋白 

分 类 号：R739.4[医药卫生—肿瘤]