瘦素受体基因多态性与原发性高血压及血脂的关系  

Association of Leptin Receptor Gene Polymorphisms with Essential Hypertension and Plasma Lipid Levels

作　　者：蔡泽园[1] 赵福梅[1] 刘婷[1] 毛用敏[1] 崔让庄[1] 

机构地区：[1]天津市胸科医院心血管病研究所,300051

出　　处：《天津医药》2011年第3期196-198,共3页Tianjin Medical Journal

摘　　要：目的:探讨瘦素受体(LEPR)基因多态性与血脂水平及原发性高血压(EH)的关系。方法:对170例原发性高血压患者(EH组)与77例正常体检者(对照组)进行研究,采用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)分析LEPR基因中第4外显子A109G和第6外显子A223G基因型和等位基因频率的分布。自动生化分析仪检测血浆多项血脂水平。结果:EH组与对照组比较,LEPRA109G基因型与等位基因频率分布差异有统计学意义(χ2分别为5.258和3.997,均P<0.05);LEPRA223G基因型与等位基因频率分布差异亦有统计学意义(χ2分别为5.454和5.146,均P<0.05)。LEPR109位和LEPR223位AG基因型携带者与GG基因型携带者相比,血浆总胆固醇、三酰甘油水平差异均无统计学意义(均P>0.05)。结论:LEPR基因第4外显子109位及第6外显子223位的基因变异与原发性高血压的发生存在一定关系。LEPR基因109位及223位不同基因型携带者血脂水平没有明显差异。Objective：To investigate the relationship between leptin receptor（LEPR）gene polymorphisms and the serum level of lipids in essential hypertensive（EH）patients. Methods：One hundred and seventy subjects with EH and 77 controls without EH were involved in this study. The LEPR A109G and LEPR A223G genotypes were determined by polymerase chain reaction（PCR）and restriction fragment length polymorphism（RFLP）. Results： Significant differences were detected in genotypes and allele frequencies of A109G and A223G polymorphisms between EH subjects and controls （P〈0.05）. The plasma levels of total cholesterol （TC） and triglyceride （TG） were no significant differences in subjects with AG genotype and GG genotype（P〉0.05）. Conclusion： LEPR gene mutation in 109 site and 223 site of exon 4 and 6 may be related with the EH. There were no obvious relationships between LEPR A109G, A223G polymorphisms and high levels of TC and TG.

关 键 词：高血压 受体 瘦素 聚合酶链反应 多态现象 遗传 等位基因 基因频率 脂类 

分 类 号：R544.1[医药卫生—心血管疾病]