胶滴药敏试验评估不同分化胃癌细胞株对化疗药物敏感性的研究  

The Use of Collagen Gel Droplet Embedded-Culture Drug Sensitivity Test in Different Gastric Cancer Cell Lines

作　　者：张建良[1] 谭志军[2] 姜伟[2] 

机构地区：[1]天津医科大学一中心临床学院,300192 [2]天津市第一中心医院普外科

出　　处：《天津医药》2011年第3期233-235,290,共4页Tianjin Medical Journal

基　　金：天津市卫生局科技基金项目(项目编号:07KY30)

摘　　要：目的:利用胶滴药敏试验评估人胃中分化腺癌细胞株SGC-7901、人胃低分化腺癌细胞株BGC-823和人胃黏液腺癌细胞株MGC-803对化疗药物的敏感性。方法:利用3个胃癌细胞株建立胶滴药敏试验,每个细胞株用顺铂、奥沙利铂、5-氟尿嘧啶(FU)、依托泊苷、阿霉素、丝裂霉素6种化疗药物测定胃癌细胞的敏感性和抑制率。结果:6种化疗药在标准浓度即100%测试药物浓度(TDC)下对人胃黏液腺癌细胞株MGC-803的抑制率分别为58.68%、63.85%、57.60%、28.09%、23.18%和40.18%,对人胃低分化腺癌细胞株BGC-823的抑制率分别为59.09%、61.26%、56.30%、33.21%、33.26%和41.20%,对胃中分化腺癌细胞株SGC-7901的抑制率分别为61.25%、69.76%、60.78%、35.00%、30.08%和58.80%。结论:体外胶滴药敏试验是一种较好的药敏检测方法,对化疗药物的体外筛选和个体化治疗具有实际应用价值。Objective： To evaluate the sensitivity of three different gastric cancer cell lines to the chemotherapy drugs with the use of the collagen gel droplet embedded-culture drug sensitivity test （CD-DST）. Methods： Three gastric cancer cell lines of SGC-7901, BGC-823 and MGC-803 were utilized to establish CD-DST. Six chemotherapy drugs, including cisplatin, oxaliplatin, 5-fluorouracil （FU）, etoposide, adriacin and mitomycin were used to measure the sensitivity and inhibition ratio in each of cancer cell lines. Results： Under standard concentration （TDC, 100% test drug concentration）, the inhibition ratios of six chemotherapy drugs to the human gastric mucous adenocarcinoma cell line MGC-803 were 58.68%, 63.85%, 57.60%, 28.09%, 23.18% and 40.18%, to the poorly differentiated human gastric adenocarcinoma cell line BGC-823 were 59.09%, 61.26%, 56.30%, 33.21%, 33.26% and 41.20%, and to moderately differentiated adenocarcinoma cell line SGC-7901 were 61.25%, 69.76%, 60.78%, 35.00%, 30.08% and 58.80%. Conclusion：CD-DST is a better method for testing drugsensitivity , which is of practical value to extracorporeal anti-cancer drug screening and individualized treatment.

关 键 词：药物筛选试验 抗肿瘤 胃肿瘤 细胞系 肿瘤 腺癌 药物疗法 

分 类 号：R733.705[医药卫生—肿瘤]