锰超氧化物歧化酶基因多态与职业性锰中毒易感性分析  

Relationship between MnSOD polymorphism and susceptibility of chronic poisoning exposed to manganism occupationally

作　　者：蔡绍雷[1] 孙明霞 闫永建[1] 孙晓光[3] 林彬[3] 傅继华[3] 

机构地区：[1]山东省医学科学院,山东省职业卫生与职业病防治研究院,济南250062 [2]山东省卫生监督所 [3]山东省疾病预防控制中心

出　　处：《中华劳动卫生职业病杂志》2011年第3期206-208,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金项目（30771789）;国家“十一五”科技支撑项目（2006BA106B02）

摘　　要：目的探讨锰超氧化物歧化酶（MnSOD）基因多态性与职业性慢性锰中毒易感性的关系。方法采用1：2配对病例-对照研究的方法，应用聚合酶链反应-限制性片段长度多态性方法（PCR．RFLP）分析与比较了164例汉族职业性慢性锰中毒病例与328例汉族对照的MnSOD基因9Ala．Val位点的基因多态性，分析MnSOD基因多态性与职业性慢性锰中毒的关系。结果MnSOD基因9Ala-Val位点V等位基因在对照组和病例组中的分布频率分别为76．7％和87．2％，差异有统计学意义（χ2=15．225，P〈0．01，OR=2．07，95％CI=1．43～3．00）；具有VV基因型的个体发生职业性慢性锰中毒的危险性与具有非VV基因型的个体相比升高了1．30倍（OR=2．30，95％CI=1．52～3．49，P〈0．05）。结论MnSOD基因可能是职业性慢性锰中毒的易感基因，携带MnSOD基因型VV可能增加职业性慢性锰中毒的危险性。Objective To study the relationship between polymorphisms of MnSOD and the susceptibility of chronic poisoning exposed to manganism occupationally. Methods In a study of case-control, genotypes were determined by PCR-RFLP in 164 patients with chronic occupational mangamism poisoning and 328 controls with age- and sex-matched for MnSOD 9Ala-Val. Results There was a significant difference in the frequency of MnSOD 9Ala-Val at V locus mutant allele between cases and controls（χ2=15.225 ,P〈0.01,95% Cl=1.43-3.00）.Individuals with the genotype VV had a 1.30 of risk increase of occupational chronic manganism poisoning compared with the the genotype AV or AA （OR=2.30,95%CI=1.52-3.49,P〈0.05）. Conclusion The MnSOD polymorphisms may be related with the susceptibility to chronic occupational manganism poisoning, the risk of chronic occupational manganism poisoning increases in carriers with genotype VV at MnSOD 9Ala-Val locus.

关 键 词：锰 超氧化物歧化酶 多态性 单核苷酸 疾病遗传易感性 

分 类 号：R1[医药卫生—公共卫生与预防医学]