次声对豚鼠凝血功能的影响  

The effects of infrasound on the blood coagulation function of guinea pigs

作　　者：马文敏[1] 亓鹏[1] 张建中[1] 易勇[1] 陈兴明[1] 张军[1] 韩瑞刚[1] 

机构地区：[1]中国人民解放军第306医院干部病房,北京100101

出　　处：《中华劳动卫生职业病杂志》2011年第3期209-211,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的研究16Hz、120dB，16Hz、125dB次声暴露后豚鼠凝血功能的变化规律，探讨次声造成生物体循环系统损害的机制。方法将豚鼠分为对照组及16Hz、120dB，16Hz、125dB2个暴露组，每个暴露组均按暴露时间再分成1、7、14、21d4个时间亚组，每个亚组8只豚鼠。各个暴露亚组每日暴露1．5h，分别连续暴露相应的天数后抽取血样测定凝血功能相关指标及血清中一氧化氮（NO）含量。结果16Hz、125dB组凝血酶原时间（PT）、国际标准化比率（INR）分别为（31．16±3．05）s和2．53±1．21，较对照组[（21．36±0．10）s、1．65±0．07]明显延长，差异有统计学意义（P〈0．05）；血清中NO含量[（88．304±52．601）μmol／L]较对照组[（30．943±26．864）μmol／L]明显升高，差异有统计学意义（P〈0．05）。16Hz、125dB组暴露14d时PT和INR较对照组明显延长，并持续至第21天。16Hz、125dB组暴露7d时血清中NO含量较对照组明显升高，且第14天继续升高，差异均有统计学意义（P〈0．05），21天时有下降趋势。结论16Hz、125dB次声暴露后豚鼠凝血功能减退，INR及门可做为评价次声对凝血功能影响的指标。Objective To study the change of the blood coagulation function of guinea pigs exposed to 16 Hz/120 dB, 16 Hz/125 dB infrasound and to explore the mechanism of circulation system damage. Methods Seventy-two guinea pigs were divided into 3 groups： the control group, the group exposed to 16 Hz/ 120 dB infrasound for 1.5 h a day and the group exposed to 16 Hz/125 dB infrasound for 1.5 h a day. Each exposure group was divided into 4 sub-groups （8 guinea pigs a sub-group） which were exposed to infrasound for 1, 7, 14 and 21 d, respectively. The coagulation function and serum nitric oxide （NO） were measured for control group and all sub-groups after exposure to infrasound. Results The prothrombin time （PT）, international normalized ratio （INR） and serum NO of group exposed to 16 Hz/125 dB infrasound were （31.16±3.05） s, 2.53±1.21 and （88.304±52.601）μmol/L, respectively, which were significantly higher than those [（21.36±0.10） s, 1.65±0.07 and （30.943±26.864） μmol/L] of control group （P〈0.05）. PT and INR of sub-groups exposed to 16 Hz/125 dB infrasound for 14 and 21 d were significantly higher than those of control group. NO of sub-groups exposed to 16 Hz/125 dB infrasound for 1 week and 2 weeks were significantly higher than that of control group （P〈0.05）, but NO of sub-group exposed to 16 Hz/125 dB infrasound for 3 weeks decreased slightly. Conclusion The blood coagulation function of guinea pigs exposed to 16 Hz/125 dB infrasound decreased, PT and INR may be used as the indexes to assess of blood coagulation function change induced by the infrasound exposure.

关 键 词：噪声 凝血酶时间 凝血酶原时间 一氧化氮 

分 类 号：R[医药卫生]