Ca^(2+)在鼻咽癌细胞凋亡性容积减小中的作用  

Roles of Ca^(2+) in Apoptotic Volume Decrease in Nasopharyngeal Carcinoma Cells

作　　者：向静瑶[1] 胡代军[1] 孙利华[1] 杨林杰[1] 左婉红[1] 朱林燕[1] 陈丽新[1] 王立伟[1] 

机构地区：[1]暨南大学医学院,广州510632

出　　处：《肿瘤防治研究》2011年第3期250-253,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30771106;30870567;30871267);暨南大学本科生科技创新工程资助项目(cx08099)

摘　　要：目的探讨钙信号途径在凋亡性细胞容积减小中的作用。方法用凋亡诱导剂顺铂诱导低分化鼻咽癌CNE-2Z细胞发生凋亡性细胞容积减小(AVD),采用Q500MC软件控制定时拍摄固定视野活细胞图像,Scion Image图像分析软件检测细胞容积变化。结果细胞外灌流顺铂后细胞体积减小,10min后细胞体积减小差异有统计学意义。去除灌流液中的Ca2+或用钙通道阻断剂Nifedipine阻断钙通道后,延缓顺铂诱导的细胞容积减小的发生,但不能防止细胞缩小过程的出现,50min后,Nifedipine组和无Ca2+灌流液组与顺铂对照组AVD水平均无差异。结论细胞外Ca2+内流可能在触发细胞凋亡早期细胞容积减小中起重要作用。Objective To study the role of calcium signaling pathway in apoptotic volume decrease（AVD） in nasopharyngeal carcinoma cells.Methods The apoptotic inducer Cisplatin was used to induce apoptotic volume decrease in poorly differentiated nasopharyngeal carcinoma cells（CNE-2Z cells）.The software Q500MC was employed for acquisition of time-lapse images of living cells.Cell volume changes were obtained by analysis of the image analysis software Scion Image.Results Extracellular application of Cisplatin induced a decrease of cell volume.The Cisplatin-induced changes in cell volume were significant 10 min after exposed to bath solution containing Cisplatin.Depletion of calcium from the bath solution or extracellular applications of calcium channel blocker nifedipine delayed the initiation of AVD induced by Cisplatin,but not the appearance of AVD.There was no significant difference in AVD 50 min after applications of Cisplatin between control and cells treated with nifedipine or Ca2＋-free bath solution.ConclusionThe inflow of Ca2＋ across the cell membrane may play an important role in activation of AVD at the early stage of apoptosis.

关 键 词：鼻咽癌 凋亡性容积减小 Ca2+信号 

分 类 号：R739.63[医药卫生—肿瘤]