甘露糖受体在正常和炎症小鼠脑内小胶质细胞的表达  

Expression of mannose receptor on microglia in the brain of normal and inflammatory mouse

作　　者：陈芸[1] 鞠莉莉[1] 王蓓蓓[1] 曾辉[1] 徐群渊[1] 

机构地区：[1]首都医科大学北京神经科学研究所,北京市神经再生修复研究重点实验室,教育部神经变性病重点实验室,北京100069

出　　处：《解剖学报》2011年第2期145-150,共6页Acta Anatomica Sinica

基　　金：国家重点基础研究发展计划资助项目(2006CB500700);国家自然科学基金资助项目(30430280);北京市科技计划基础研究专项资助项目(Z0005187040311);2009年北京市科技新星计划(B类)资助项目(2009B05)

摘　　要：目的确定脑内小胶质细胞是否表达甘露糖受体,以及在不同脑区甘露糖受体的表达是否存在差异,以进一步明确小胶质细胞的功能。方法 C57小鼠26只,分为侧脑室炎症模型组(10只)、全身炎症模型组(6只)和正常对照组(10只)。通过注射细菌脂多糖(LPS)建立全脑急性炎症模型,用免疫荧光双标技术对小鼠脑组织冷冻切片进行染色,激光共焦显微镜下观察小胶质细胞(Iba-1阳性)或激活小胶质细胞(OX-6阳性)与甘露糖受体(CD206)的共定位,比较皮层、海马、纹状体、黑质和小脑5个脑区小胶质细胞和CD206共定位比例的差异。结果正常小鼠和急性侧脑室炎症小鼠皮层内的部分小胶质细胞(包括Iba-1阳性细胞与OX-6阳性细胞)有CD206表达,CD206阳性小胶质细胞的比例分别是83%和94%;激活小胶质细胞(表达OX-6)与CD206有共定位,CD206阳性细胞中的激活小胶质细胞在皮层、海马、纹状体、黑质和小脑的比例分别是79%、81%、74%、53%和66%,而激活小胶质细胞中的CD206阳性细胞的比例则是63%、48%、49%、53%和52%。结论脑内静息和激活的小胶质细胞都能够表达甘露糖受体,表达量受炎症因素的影响并在不同脑区存在差异,其中黑质内CD206阳性细胞中的激活小胶质细胞的比例明显低于其他脑区;激活小胶质细胞中CD206阳性细胞的比例则在海马偏低。Objective To explore the expression of mannose receptor on microglia and its difference in different brain areas in the mouse, in order to understand better the microglial function. Methods The expression of mannose receptor on microglia was revealed by using immunofluorescencent double-labeling technique either in ten normal brains （ Iba-1 ） and ten inflammatory brains induced by unilaterally cerebroventricular injection of lippolysaccharide （LPS） or six brains with a acute inflammation induced by intraperitoneal injection of LPS in C57 adult mice. The comparison of the expressions in different brain regions（ cortex, hippocampus, striatum, substantia uigra and cerebellum）was done in those normal and inflammatory mice, respectively. Results The expression of mannose receptor （CD206） showed clearly in part of microglia （ both of Iba-1 positive cells and OX-6 positive cells ） either in normal and inflammatory cortex. The CD206 positive cells were 83% and 94% in total cortical microglia, respectively. The activated microglia （OX-6 positive cells） in total CD206 positive cells were 79%, 81%, 74%, 53% and 66% in the cortex, hippocampus, striatum, substantia nigra and cerebellum, while the CD206 positive cells in activated microglia were 63% , 48% , 49% , 53% and 52% , respectively. Conclusion Either the rest or activated microglia express mannose receptors and this expression can be enhanced by brain inflammation. This expression has also a regional difference in the brain. The ratio of activated microglia in total CD206 positive cells is lower in substantia nigra than that in other brain areas, while the ratio of CD206 positive cells in activated microglia is lower in hippocampus.

关 键 词：小胶质细胞 炎症 甘露糖受体 免疫荧光 小鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]