异体脂肪源干细胞移植对糖皮质激素性骨质疏松大鼠的影响  

Effects of systemic transplantation of allograft adipose-derived stem cells in glucocorticoid-induced osteoporosis rats

作　　者：陶晖[1] 杨会营[1] 余美春[1] 杨春[1] 曾文钦[1] 段富华[1] 戴景兴[1] 原林[1] 

机构地区：[1]南方医科大学人体解剖学教研室,广州510515

出　　处：《解剖学报》2011年第2期220-225,共6页Acta Anatomica Sinica

基　　金：国家重点基础研究计划(973计划)资助项目(2007CB512705);国家自然科学基金青年面上资助项目(30801464)

摘　　要：目的基于"筋膜学假说",探索未分化非特异性结缔组织(筋膜)支架构成的支持与储备系统中的主要细胞储备成分——脂肪源干细胞(ADSCs)移植对糖皮质激素性骨质疏松大鼠骨密度及骨生物力学的影响。方法 40只Wistar雌性大鼠随机分为空白对照组、模型组、治疗组和治疗对照组。利用糖皮质激素建立骨质疏松模型后,经尾静脉移植ADSCs 3×106个/只进行治疗。测定血清Ca、P、碱性磷酸酶(ALP)的含量,L3～L5椎体和右侧股骨骨密度以及左侧股骨最大载荷和刚度。结果糖皮质激素注射12周后,模型组血清Ca和ALP、骨密度、最大载荷和刚度均明显低于空白对照组,而血清P明显升高(P<0.01)。ADSCs治疗4周后,治疗组血清Ca和ALP、骨密度、最大载荷和刚度均明显升高,而血清P水平下降。结论移植异体ADSCs可以有效改善糖皮质激素性骨质疏松大鼠的骨密度和骨生物力学性能,为糖皮质激素性骨质疏松的治疗提供了新的治疗方法,并为"筋膜学假说"提供了部分实验依据。Objective To investigate the effects of systemic transplantation of a11ograft adipose-derived stem cells （ADSCs） , which are mainly undifferentiated stem cells in the supporting and storing system consisting of the non-specific connective tissue （fascia） stent, on the bone mineral density （BMD） and bone biomechanics in glucocorticoid-induced osteoporosis （GIOP） rats based on fasciaology hypothesis, and to explore a new therapeutics for osteoporosis. Methods Totally 40 female aduh Wistar rats were randomly divided into four groups： blank control group （A）, model group （B）, treatment group（C） and treatment control group（D） （n = 10）. Rats were induced osteoporosis by injected prednisolone. Then each rat was treated with ADSCs 3- 106 via tail vein injection. BMD of L3-L5 and right femurs,strength and stiffness of left femurs, the levels of serum Ca,P and alkaline phosphatase（ALP） were detected. Results Prednisolone injection for 12 weeks, the levels of serum Ca and ALP, the BMD of L3-L5 and right femurs, the strength and stiffness of left femurs in B group were obviously lower than that of A group, while serum P levels higher（P 〈0. 01 ）. ADSCs after 4 weeks of treatment, in C group the levels of serum Ca and ALP, BMD, the strength and stiffness were significantly increased, and the serum P levels decreased. Conclusion Transplanted allograft ADSCs could improve the BMD and bone biomechanics of GIOP rats. The systemic transplantation of ADSCs could be considered as a new treatment for GIOP,and this also could provide some basis for fasciaology hypothesis.

关 键 词：脂肪源干细胞 骨质疏松 糖皮质激素 骨密度 筋膜学 大鼠 

分 类 号：R394.2[医药卫生—医学遗传学]