HIV-1感染对细胞内宿主因子TSG101及与Alix表达的影响  

HIV-1 Infection Affects the Expression of Host Cell Factor TSG101 and Alix

作　　者：胡辉亮[1] 孟哲峰[2] 张晓燕[2,3] 吕建新[1] 

机构地区：[1]温州医学院浙江省医学遗传学重点实验室检验医学院暨生命科学学院,温州325000 [2]复旦大学上海市公共卫生临床中心生物医学研究院,上海201508 [3]中国疾病预防控制中心传染病预防控制国家重点实验室,北京102206

出　　处：《病毒学报》2011年第2期129-134,共6页Chinese Journal of Virology

基　　金：国家自然基金青年项目(30901257);中国博士后基金第三批特别资助项目(201003233);国家十一五新药(2009ZX09303-006);国家十一五传染病研究重大专项(2008ZX10001-015)资助

摘　　要：探讨HIV-1感染宿主细胞后对其宿主蛋白肿瘤易感基因101蛋白(Tumor Susceptibility Gene 101,TSG101)及ALG-2相互作用蛋白X(ALG-2-interacting protein X,Alix)表达的影响。以HIV-1感染性克隆病毒pNL4-3感染TZM-bl PM1、Jurkat细胞株和人外周血单个核细胞(PBMCs),感染24h后收获细胞提取总RNA,逆转录PCR检测在RNA水平各因子的表达差异;感染48h后收获细胞提取总蛋白,Western-blot检测各因子在蛋白水平的表达差异。结果显示:HIV-1感染对原代PBMC与细胞系表达Alix与TSG101影响显著不同,细胞系主要表现为下调,而原代PBMC主要表现为TSG101上调;细胞系中的下调又细分为Jurkat细胞的Alix与TSG101的双下调、TZM-bl细胞的Alix单下调以及PM1细胞无影响三种情况。HIV-1感染对细胞宿主分子TSG101及Alix在RNA和蛋白水平的表达均有影响,这种影响因细胞的不同而有差异。HIV-1感染调节Alix与TSG101的机制生物学意义尚有待于进一步阐明。To investigate the effects of HIV-1 infection on the expression of host factors TSG101（Tumor Susceptibility Gene 101） and Alix（ALG-2-interacting protein X）.HIV-1 infectious clone pNL4-3 was used to infect TZM-bl,PM1,Jurkat cell lines and human peripheral blood mononuclear cells（PBMC）.Twenty-four hours post-infection,the infected or uninfected cells were harvested respectively for extraction of total RNAs and total cellular proteins,which were subsequently used in RT-PCR and Western-blotting respectively to quantify TSG101 and Alix,respectively.Our data showed that HIV-1 infection resulted in various influences on the expression of TSG101 and Alix in the cell lines and the primary PBMC.A down-regulation was mainly observed in the cell lines,whereas an up-regulation of TSG101 was identified in primary PBMC.Three patterns were observed for down-regulation,including dual down-regulation of TSG101 and Alix for Jurkat cells,single down-regulation of Alix for TZM-bl cells and marginal or no influence on PM1 cells.The dual down-regulation of Alix and TSG101 in Jurkat cells coincided with less expression of HIV-1 p24 protein.This is the first-line evidence that HIV-1 infection affects the expression of host factors TSG101 and Alix,the down-regulation of these molecules may influence the HIV-1 replication.The underlying mechanism remains to be addressed.

关 键 词：HIV-1 感染 TSG101 ALIX 

分 类 号：R373.9[医药卫生—病原生物学] R512.91[医药卫生—基础医学]