Notch-1、NF-kB和β-catenin在口腔鳞状细胞癌中的表达及其临床意义  

Expression and clinicopathological significance of Notch-1,NF-kB and β-catenin in oral squamous cell carcinoma

作　　者：李雪珍[1] 金晓明[1] 刘民生 

机构地区：[1]青岛大学医学院口腔颌面外科,山东青岛266011 [2]莱芜市中医医院耳鼻喉科,山东莱芜271100

出　　处：《山东大学耳鼻喉眼学报》2011年第1期9-13,共5页Journal of Otolaryngology and Ophthalmology of Shandong University

摘　　要：目的通过观察Notch-1、NF-kB和β-catenin在口腔鳞状细胞癌中的表达情况,探讨Notch-1、NF-kB及Wnt/1β-catenin信号传导系统与口腔鳞状细胞癌发生、发展的相关性。方法应用免疫组织化学染色方法检测58例口腔鳞状细胞癌组织及30例癌周组织中Notch-1、NF-kB和β-catenin蛋白表达情况并分析其与临床病理特征的关系。结果①Notch-1、NF-kB蛋白在口腔鳞状细胞癌组织中的表达明显高于在癌周组织,并且两者染色积分间的差异有统计学意义(P<0.01)。两种蛋白的表达水平与患者的年龄、性别、肿瘤原发部位和组织学分级之间无相关性,但是与肿瘤TNM分期以及有无淋巴结转移密切相关(P<0.01);②在58例口腔鳞状细胞癌组织中β-cate-nin异位表达23例(79.31%),β-catenin异位表达与肿瘤TNM分期(P<0.01)以及有无淋巴结转移密切相关(P<0.05);③Spearman等级相关分析口腔鳞状细胞癌组织中Notch-1、NF-kB表达和β-catenin异位表达之间的相关性,Notch-1和NF-kB表达二者表达有显著关联性(P<0.05);Notch-1表达和β-catenin异位表达差异有统计学意义(P<0.05);而NF-kB蛋白表达和β-catenin异位表达未见明显相关性(P>0.05)。结论 Notch-1、NF-ΚB高表达和β-catenin异位表达在口腔鳞状细胞癌中属常见现象,并且三者表达情况均与肿瘤TNM分期以及有无淋巴结转移密切相关。Notch-1表达分别和NF-kB表达及β-catenin异位表达差异有统计学意义,但NF-kB表达和β-catenin异位表达差异无统计学意义。Objective To investigate expressions of Notch-1,NF-kB and β-catenin in oral squamous cell carcinoma,and explore their associations with the clinicopathological features.Methods Expressions of Notch-1,NF-kB and β-catenin were analyzed in 58 oral squamous cell carcinoma samples and 30 normal tissues adjacent to the carcinomas by immunohistochemistry.Results a） Notch-1 and NF-kB protein were up-regulated in most oral squamous cell carcinoma samples but not in normal tissues.The difference of weighted scores between these two groups was significant（P〈0.01）.Although there were no correlations between the expressions of Notch-1,NF-kB protein and some tumor parameters,such as age,gender,tumor site and histological grade,expressions of Notch-1,NF-kB protein were significantly associated with clinical staging（P〈0.01） and lymph node metastasis（P〈0.05）.b） Higher abnormalβ-catenin expression was noted in most oral squamous cell carcinoma samples than in normal tissues.The difference of weighted scores between these two group was also significant（P〈0.01）.There were no correlations between expressions of abnormalβ-catenin expression and some tumor parameters,such as age,gender,tumor site and histological grade,while expression of abnormalβ-catenin was significantly associated with clinical staging（P〈0.01） and lymph node metastasis（P〈0.05）.c） In oral squamous carcinoma tissues,there were correlation between expression of Notch-1 and NF-kB,and there were also correlation between expression of Notch-1 and ectopic expression of β-catenin（P〈0.05）.But there was no correlation between expression of NF-kB and ectopic expression of β-catenin（P〉0.05）.Conclusions Over-expression of Notch-1,NF-kB or ectopic expression of β-catenin expression are common in oral squamous cell carcinoma.Expression of Notch-1 and NF-kB or ectopic expression of β-catenin expression are significantly correlated with tumor staging and lymph node metastasis.Expression of Notch-1 were correlatied with

关 键 词：口腔鳞状细胞癌 NOTCH-1 NF—KB Β-CATENIN 表达 

分 类 号：R739.8[医药卫生—肿瘤]